| Texto completo | |
| Autor(es): |
Biselli, J. M.
;
Goloni-Bertollo, E. M.
;
Zampieri, B. L.
;
Haddad, R.
;
Eberlin, M. N.
;
Pavarino-Bertelli, E. C.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Genetics and Molecular Research; v. 7, n. 1 2008. |
| Área do conhecimento: | Ciências da Saúde - Medicina |
| Assunto(s): | Síndrome de Down Polimorfismo genético |
| Resumo | |
The aim of the present study was to investigate the effect of polymorphisms C677T and A1298C in the methylenetetrahydrofolate reductase (MTHFR) gene, A2756G in methionine synthase reductase (MTR) gene and A80G in reduced folate carrier 1 (RFC1) gene, and plasma homocysteine (Hcy), on the maternal risk for Down syndrome (DS). Seventy-two DS mothers and 194 mothers who had no children with DS were evaluated. The investigation of the MTHFR C677T, MTR A2756G and RFC1 A80G polymorphisms was performed by polymerase chain reaction and enzyme digestion and the MTHFR A1298C polymorphism by allele-specific polymerase chain reaction. Hcy quantification was carried out by liquid chromatography-tandem mass spectrometry. The median number of polymorphic alleles for the four loci tested was greater in DS mothers compared to the control group, and the presence of three or more polymorphic alleles increased the risk for having a child with DS 1.74 times. Elevated maternal risk for DS was also observed when plasma Hcy concentration was higher than 4.99 mu mol/L. In conclusion, the presence of three or more polymorphic alleles for MTHFR C677T, MTHFR A1298C, MTR A2756G, and RFC1 A80G, and plasma Hcy concentrations higher than 4.99 mu mol/L are maternal risk factors for DS. (AU) | |
| Processo FAPESP: | 04/15944-5 - Avaliação genético-clínica e molecular em Síndrome de Down |
| Beneficiário: | Erika Cristina Pavarino |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 05/60883-7 - Investigacao do polimorfismo c677t do gene metilenotetrahidrofolato redutase (mthfr) como fator de risco materno para a sindrome de down. |
| Beneficiário: | Geisa Colebrusco de Souza Gonçalves |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |