Leucine supplementation improves regeneration of skeletal muscles from old rats

Autor(es)

Pereira, Marcelo G. ^[1] ; Silva, Meiricris T. ^[1] ; da Cunha, Fernanda M. ^[2] ; Moriscot, Anselmo S. ^[1] ; Aoki, Marcelo S. ^[3] ; Miyabara, Elen H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-0550800 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Biochem, Vila Clementino, BR-04044020 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-03828000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Gerontology; v. 72, p. 269-277, DEC 2015.
• Citações Web of Science: 15

Resumo

The decreased regenerative capacity of old skeletal muscles involves disrupted turnover of proteins. This study investigated whether leucine supplementation in old rats could improve muscle regenerative capacity. Young and old male Wistar rats were supplemented with leucine; then, the muscles were cryolesioned and examined after 3 and 10 days. Leucine supplementation attenuated the decrease in the expression of eukaryotic translation initiation factor 4E binding protein 1 (4E-BPI) and eukaryotic translation initiation factor 4E (elF4E) in young and old muscles on day 3 post-injury and promoted an increase in the cross-sectional area of regenerating myofibers from both young and old soleus muscles on clay 10 post-injury. This supplementation decreased the levels of ubiquitinated proteins and increased the proteasome activity in young regenerating muscles, but the opposite effect was observed in old regenerating muscles. Moreover, leucine decreased the inflammation area and induced an increase in the number of proliferating satellite cells in both young and old muscles. Our results suggest that leucine supplementation improves the regeneration of skeletal muscles from old rats, through the preservation of certain biological responses upon leucine supplementation. Such responses comprise the decrease in the inflammation area, increase in the number of proliferating satellite cells and size of regenerating myofibers, combined with the modulation of components of the phosphoinositide 3-kinase/Akt-protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway and ubiquitin-proteasome system. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/50688-3 - Efeitos da restrição calórica sobre o sistema ubiquitina-proteassomo durante o envelhecimento de leveduras: mecanismos dependentes de pH e papel de heat shock proteins
• Beneficiário: Fernanda Marques da Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/15276-9 - Plasticidade muscular esquelética após lesão em ratos idosos: efeito da suplementação com leucina
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/52520-0 - Efeito da suplementacao com leucina no processo de regeneracao muscular esqueletica.
• Beneficiário: Marcelo Gomes Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/54240-7 - Papel do adrenocpetor beta 2 na regeneracao muscular esqueletica
• Beneficiário: Meiricris Tomaz da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/50500-7 - Estudo do envolvimento da sinalização retrógrada RTG-dependente na manutenção da atividade mitocondrial durante o envelhecimento de Saccharomyces cerevisiae
• Beneficiário: Fernanda Marques da Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/13874-1 - Regeneração muscular esquelética de camundongos com Síndrome de Marfan: estudo do potencial regenerativo das células satélites
• Beneficiário: Meiricris Tomaz da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs