| Full text | |
| Author(s): |
Pereira, Marcelo G.
[1]
;
Silva, Meiricris T.
[1]
;
da Cunha, Fernanda M.
[2]
;
Moriscot, Anselmo S.
[1]
;
Aoki, Marcelo S.
[3]
;
Miyabara, Elen H.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-0550800 Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biochem, Vila Clementino, BR-04044020 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-03828000 Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Experimental Gerontology; v. 72, p. 269-277, DEC 2015. |
| Web of Science Citations: | 15 |
| Abstract | |
The decreased regenerative capacity of old skeletal muscles involves disrupted turnover of proteins. This study investigated whether leucine supplementation in old rats could improve muscle regenerative capacity. Young and old male Wistar rats were supplemented with leucine; then, the muscles were cryolesioned and examined after 3 and 10 days. Leucine supplementation attenuated the decrease in the expression of eukaryotic translation initiation factor 4E binding protein 1 (4E-BPI) and eukaryotic translation initiation factor 4E (elF4E) in young and old muscles on day 3 post-injury and promoted an increase in the cross-sectional area of regenerating myofibers from both young and old soleus muscles on clay 10 post-injury. This supplementation decreased the levels of ubiquitinated proteins and increased the proteasome activity in young regenerating muscles, but the opposite effect was observed in old regenerating muscles. Moreover, leucine decreased the inflammation area and induced an increase in the number of proliferating satellite cells in both young and old muscles. Our results suggest that leucine supplementation improves the regeneration of skeletal muscles from old rats, through the preservation of certain biological responses upon leucine supplementation. Such responses comprise the decrease in the inflammation area, increase in the number of proliferating satellite cells and size of regenerating myofibers, combined with the modulation of components of the phosphoinositide 3-kinase/Akt-protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway and ubiquitin-proteasome system. (C) 2015 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 12/15276-9 - Skeletal muscle plasticity after injury in old rats: effect of leucine supplementation |
| Grantee: | Elen Haruka Miyabara |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/50500-7 - Involvement of the RTG-dependent retrograde signaling pathway in the maintenance of mitochondrial activity during ageing in Saccharomyces cerevisiae |
| Grantee: | Fernanda Marques da Cunha |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/13874-1 - Skeletal muscle regeneration in mice with Marfan Syndrome: study of the regenerative potential of satellite cells |
| Grantee: | Meiricris Tomaz da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |