Exercise training restores the cardiac microRNA-1 and-214 levels regulating Ca2+ handling after myocardial infarction

Autor(es)

Soares Melo, Stephano Freitas ^[1] ; Barauna, Valerio Garrone ^[2] ; Neves, Vander Jose ^[1] ; Fernandes, Tiago ^[1] ; Lara, Lucienne da Silva ^[3] ; Mazzotti, Diego Robles ^[4] ; Oliveira, Edilamar Menezes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Lab Biochem & Mol Biol Exercise, Sao Paulo - Brazil

^[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Lab Mol Physiol, Vitoria - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Biomed Sci, Rio De Janeiro - Brazil

^[4] Univ Fed Sao Paulo, Dept Hlth Informat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC CARDIOVASCULAR DISORDERS; v. 15, DEC 9 2015.
• Citações Web of Science: 24

Resumo

Background: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and -214 expression after myocardial infarction. Methods: Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction. Results: MicroRNA-1 and -214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group. Conclusions: These results suggest that exercise training restores microRNA-1 and -214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats. (AU)

• Processo FAPESP: 09/18370-3 - Expressão de microRNAs no coração de ratos normotensos submetidos a diferentes volumes de treinamento físico aeróbico e seu potencial terapêutico na hipertensão
• Beneficiário: Edilamar Menezes de Oliveira
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/09438-0 - Utilização terapêutica de microRNAs responsáveis pela regulação da contratilidade cardíaca associado ao treinamento físico aeróbico em ratos com insuficiência cardíaca
• Beneficiário: Stéphano Freitas Soares Melo
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/04104-2 - Expressão de microRNAs na aorta torácica de ratos normotensos submetidos a treinamento físico aeróbio: perspectivas de identificação de alvos terapêuticos relacionados à hipertensão arterial
• Beneficiário: Vander José das Neves
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/50048-1 - Bases celulares e funcionais do exercício físico na doença cardiovascular
• Beneficiário: Carlos Eduardo Negrão
• Linha de fomento: Auxílio à Pesquisa - Temático