| Texto completo | |
| Autor(es): |
Soares Melo, Stephano Freitas
[1]
;
Barauna, Valerio Garrone
[2]
;
Neves, Vander Jose
[1]
;
Fernandes, Tiago
[1]
;
Lara, Lucienne da Silva
[3]
;
Mazzotti, Diego Robles
[4]
;
Oliveira, Edilamar Menezes
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Phys Educ & Sport, Lab Biochem & Mol Biol Exercise, Sao Paulo - Brazil
[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Lab Mol Physiol, Vitoria - Brazil
[3] Univ Fed Rio de Janeiro, Inst Biomed Sci, Rio De Janeiro - Brazil
[4] Univ Fed Sao Paulo, Dept Hlth Informat, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC CARDIOVASCULAR DISORDERS; v. 15, DEC 9 2015. |
| Citações Web of Science: | 24 |
| Resumo | |
Background: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and -214 expression after myocardial infarction. Methods: Wistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction. Results: MicroRNA-1 and -214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group. Conclusions: These results suggest that exercise training restores microRNA-1 and -214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats. (AU) | |
| Processo FAPESP: | 10/50048-1 - Bases celulares e funcionais do exercício físico na doença cardiovascular |
| Beneficiário: | Carlos Eduardo Negrão |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 10/09438-0 - UTILIZAÇÃO TERAPÊUTICA DE microRNAS RESPONSÁVEIS PELA REGULAÇÃO DA CONTRATILIDADE CARDÍACA ASSOCIADO AO TREINAMENTO FÍSICO AERÓBICO EM RATOS COM INSUFICIÊNCIA CARDÍACA |
| Beneficiário: | Stéphano Freitas Soares Melo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/04104-2 - Expressão de microRNAs na aorta torácica de ratos normotensos submetidos a treinamento físico aeróbio: perspectivas de identificação de alvos terapêuticos relacionados à hipertensão arterial. |
| Beneficiário: | Vander José das Neves |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 09/18370-3 - EXPRESSÃO DE microRNAS NO CORAÇÃO DE RATOS NORMOTENSOS SUBMETIDOS A DIFERENTES VOLUMES DE TREINAMENTO FÍSICO AERÓBICO E SEU POTENCIAL TERAPÊUTICO NA HIPERTENSÃO |
| Beneficiário: | Edilamar Menezes de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |