Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures

Autor(es)

Caron, Angelo Luis ^[1] ; Picanco-Castro, Virginia ^[2] ; Ansorge, Sven ^[3] ; Covas, Dimas Tadeu ^[2] ; Kamen, Amine ^[4] ; Swiech, Kamilla ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP - Brazil

^[2] Hemoctr Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2 - Canada

^[4] McGill Univ, Dept Bioengn, Montreal, PQ - Canada

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 58, n. 6, p. 923-928, NOV-DEC 2015.
• Citações Web of Science: 1

Resumo

ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles. (AU)

• Processo FAPESP: 12/04629-8 - Estabelecimento de uma plataforma de produção de proteínas recombinantes terapêuticas em células humanas
• Beneficiário: Kamilla Swiech Antonietto
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs