Establishment of a production platform for recombinant therapeutic proteins in hum...
| Full text | |
| Author(s): |
Caron, Angelo Luis
[1]
;
Picanco-Castro, Virginia
[2]
;
Ansorge, Sven
[3]
;
Covas, Dimas Tadeu
[2]
;
Kamen, Amine
[4]
;
Swiech, Kamilla
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP - Brazil
[2] Hemoctr Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2 - Canada
[4] McGill Univ, Dept Bioengn, Montreal, PQ - Canada
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Brazilian Archives of Biology and Technology; v. 58, n. 6, p. 923-928, NOV-DEC 2015. |
| Web of Science Citations: | 1 |
| Abstract | |
Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x10(8) particles. (AU) | |
| FAPESP's process: | 12/04629-8 - Establishment of a production platform for recombinant therapeutic proteins in human cells |
| Grantee: | Kamilla Swiech Antonietto |
| Support type: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/08135-2 - CTC - Center for Cell-Based Therapy |
| Grantee: | Dimas Tadeu Covas |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |