Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures

Author(s)

Caron, Angelo Luis ^[1] ; Picanco-Castro, Virginia ^[2] ; Ansorge, Sven ^[3] ; Covas, Dimas Tadeu ^[2] ; Kamen, Amine ^[4] ; Swiech, Kamilla ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP - Brazil

^[2] Hemoctr Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2 - Canada

^[4] McGill Univ, Dept Bioengn, Montreal, PQ - Canada

• Document type: Journal article
• Source: Brazilian Archives of Biology and Technology; v. 58, n. 6, p. 923-928, NOV-DEC 2015.
• Web of Science Citations: 1

Abstract

ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles. (AU)

• FAPESP's process: 12/04629-8 - Establishment of a production platform for recombinant therapeutic proteins in human cells
• Grantee: Kamilla Swiech Antonietto
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC