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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures

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Caron, Angelo Luis [1] ; Picanco-Castro, Virginia [2] ; Ansorge, Sven [3] ; Covas, Dimas Tadeu [2] ; Kamen, Amine [4] ; Swiech, Kamilla [1]
Total Authors: 6
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP - Brazil
[2] Hemoctr Ribeirao Preto, Ribeirao Preto, SP - Brazil
[3] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2 - Canada
[4] McGill Univ, Dept Bioengn, Montreal, PQ - Canada
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Archives of Biology and Technology; v. 58, n. 6, p. 923-928, NOV-DEC 2015.
Web of Science Citations: 1

Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x10(8) particles. (AU)

FAPESP's process: 12/04629-8 - Establishment of a production platform for recombinant therapeutic proteins in human cells
Grantee:Kamilla Swiech Antonietto
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC