Antibodies are not required to a protective immune response against dengue virus elicited in a mouse encephalitis model

Autor(es)

Amorim, Jaime Henrique ^[1] ; dos Santos Alves, Rubens Prince ^[1] ; Bizerra, Raiza ^[1] ; Pereira, Sara Araujo ^[1] ; Pereira, Lennon Ramos ^[1] ; Nascimento Fabris, Denicar Lina ^[1] ; Santos, Robert Andreata ^[1] ; Romano, Camila Malta ^{[2, 3]} ; de Souza Ferreira, Luis Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Microbiol, Lab Desenvolvimento Vacinas, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Mol Infecciosas & Parasitarias LIMHC, Inst Med Trop Sao Paulo, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY; v. 487, p. 41-49, JAN 2016.
• Citações Web of Science: 8

Resumo

Generating neutralizing antibodies have been considered a prerequisite to control dengue virus (DENV) infection. However, T lymphocytes have also been shown to be important in a protective immune state. In order to investigate the contribution of both humoral and cellular immune responses in DENV immunity, we used an experimental model in which a non-lethal DENV2 strain (ACS46) is used to intracranially prime Balb/C mice which develop protective immunity against a lethal DENV2 strain (JHA1). Primed mice generated envelope-specific antibodies and CD8(+) T cell responses targeting mainly non-structural proteins. Immune sera from protected mice did not confer passive protection to naive mice challenged with the JHA1 strain. In contrast, depletion of CD4(+) and CD8(+) T lymphocytes significantly reduced survival of ACS46-primed mice challenged with the JHA1 strain. Collectively, results presented in this study show that a cellular immune response targeting non-structural proteins are a promising way in vaccine development against dengue. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/51204-2 - Um novo caminho para a busca racional de uma vacina efetiva contra a dengue
• Beneficiário: Jaime Henrique Amorim Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado