Characterization of reactive stroma in prostate cancer: involvement of growth factors, metalloproteinase matrix, sexual hormones receptors and prostatic stem cells

Autor(es)

da Silva Junior, Mauricio Moreira ^[1] ; Matheus, Wagner Eduardo ^[1] ; Garcia, Patrick Vianna ^[2] ; Stopiglia, Rafael Mamprim ^[1] ; Billis, Athanase ^[3] ; Ferreira, Ubirajara ^[1] ; Favaro, Wagner Jose ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Area Urooncol, Dept Cirurgia, BR-13083865 Campinas, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Dept Biol Estrutural & Func, Lab Carcinogenese Urogenital & Imunoterapia LCURG, BR-13083865 Campinas, SP - Brazil

^[3] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Dept Patol, BR-13083865 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL BRAZ J UROL; v. 41, n. 5, p. 849-858, SEP-OCT 2015.
• Citações Web of Science: 4

Resumo

Introduction and Objectives: Reactive Stroma (RStr) is observed in many human cancers and is related to carcinogenesis. The objectives of the present study were to stablish a relationship of the RStr microenvironment with prostate cancer (Pca) through a morphological and molecular characterization, and to identify a possible relationship between RStr with worse prognosis factors and occurrence of malignant prostatic stem cells. Materials and Methods: Forty prostatic samples were selected from men with Pca diagnosis submitted to radical prostatectomy; they were divided in two groups: Group-1 (n = 20): samples without reactive stroma; Group-2 (n = 20): samples of PCa with intense stroma reaction. Prostatic samples were evaluated for RStr intensity by Masson Trichromic stain and posteriorly submitted to histopathological and immunohistochemistry analysis for antigens: alpha-actin, vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA, AR, Er alpha and ER beta. Results: Reactive stroma with intense desmoplastic reactivity was significantly more frequent in intermediate (Gleason 7, 3+4) and high grade tumors (Gleason 7, 4+3). The group with intense stromal reactivity showed significant higher levels of Vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA and ER alpha. Conclusions: It can be concluded that RStr may be a predictive marker of Pca progression, since it was associated with increase of growth factors, imbalance of androgen and estrogen receptors and presence of malign prostatic stem cells. (AU)

• Processo FAPESP: 09/50397-9 - Celulas-tronco prostaticas: distribuicao especifica nos compartimentos epitelial e estromal da prostata ventral de ratos senis frente a diferentes condicoes hormonais e na prostata de homens senis com e..
• Beneficiário: Wagner José Fávaro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado