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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of reactive stroma in prostate cancer: involvement of growth factors, metalloproteinase matrix, sexual hormones receptors and prostatic stem cells

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Author(s):
da Silva Junior, Mauricio Moreira [1] ; Matheus, Wagner Eduardo [1] ; Garcia, Patrick Vianna [2] ; Stopiglia, Rafael Mamprim [1] ; Billis, Athanase [3] ; Ferreira, Ubirajara [1] ; Favaro, Wagner Jose [2]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Area Urooncol, Dept Cirurgia, BR-13083865 Campinas, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Dept Biol Estrutural & Func, Lab Carcinogenese Urogenital & Imunoterapia LCURG, BR-13083865 Campinas, SP - Brazil
[3] Univ Estadual Campinas UNICAMP, Fac Ciencias Med, Dept Patol, BR-13083865 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INTERNATIONAL BRAZ J UROL; v. 41, n. 5, p. 849-858, SEP-OCT 2015.
Web of Science Citations: 4
Abstract

Introduction and Objectives: Reactive Stroma (RStr) is observed in many human cancers and is related to carcinogenesis. The objectives of the present study were to stablish a relationship of the RStr microenvironment with prostate cancer (Pca) through a morphological and molecular characterization, and to identify a possible relationship between RStr with worse prognosis factors and occurrence of malignant prostatic stem cells. Materials and Methods: Forty prostatic samples were selected from men with Pca diagnosis submitted to radical prostatectomy; they were divided in two groups: Group-1 (n = 20): samples without reactive stroma; Group-2 (n = 20): samples of PCa with intense stroma reaction. Prostatic samples were evaluated for RStr intensity by Masson Trichromic stain and posteriorly submitted to histopathological and immunohistochemistry analysis for antigens: alpha-actin, vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA, AR, Er alpha and ER beta. Results: Reactive stroma with intense desmoplastic reactivity was significantly more frequent in intermediate (Gleason 7, 3+4) and high grade tumors (Gleason 7, 4+3). The group with intense stromal reactivity showed significant higher levels of Vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA and ER alpha. Conclusions: It can be concluded that RStr may be a predictive marker of Pca progression, since it was associated with increase of growth factors, imbalance of androgen and estrogen receptors and presence of malign prostatic stem cells. (AU)