The Pangenome of the Anticarsia gernmatalis Multiple Nucleopolyhedrovirus (AgMNPV)

Autor(es)

de Brito, Anderson Fernandes ^[1] ; Braconi, Carla Torres ^[1] ; Weidmann, Manfred ^[2] ; Dilcher, Meik ^[2] ; Pereira Alves, Joao Marcelo ^[3] ; Gruber, Arthur ^[3] ; de Andrade Zanotto, Paolo Marinho ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Mol Evolut & Bioinformat, Inst Biomed Sci ICB 2, Dept Microbiol, Sao Paulo, SP - Brazil

^[2] Univ Med Ctr, Dept Virol, Gottingen - Germany

^[3] Univ Sao Paulo, Dept Parasitol, Inst Biomed Sci ICB 2, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENOME BIOLOGY AND EVOLUTION; v. 8, n. 1, p. 94-108, JAN 2016.
• Citações Web of Science: 4

Resumo

The alphabaculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) is the world's most successful viral bioinsecticide. Through the 1980s and 1990s, this virus was extensively used for biological control of populations of Anticarsia gemmatalis (Velvetbean caterpillar) in soybean crops. During this period, genetic studies identified several variable loci in the AgMNPV; however, most of them were not characterized at the sequence level. In this study we report a full genome comparison among 17 wild-type isolates of AgMNPV. We found the pangenome of this virus to contain at least 167 hypothetical genes, 151 of which are shared by all genomes. The gene bro-a that might be involved in host specificity and carrying transporter is absent in some genomes, and new hypothetical genes were observed. Among these genes there is a unique rnf12-like gene, probably implicated in ubiquitination. Events of gene fission and fusion are common, as four genes have been observed as single or split open reading frames. Gains and losses of genomic fragments (from 20 to 900 bp) are observed within tandem repeats, such as in eight direct repeats and four homologous regions. Most AgMNPV genes present low nucleotide diversity, and variable genes are mainly located in a locus known to evolve through homologous recombination. The evolution of AgMNPV is mainly driven by small indels, substitutions, gain and loss of nucleotide stretches or entire coding sequences. These variations may cause relevant phenotypic alterations, which probably affect the infectivity of AgMNPV. This work provides novel information on genomic evolution of the AgMNPV in particular and of baculoviruses in general. (AU)

• Processo FAPESP: 11/17120-3 - Análise da diversidade genética de populações do nucleopoliedrovírus Anticarsia gemmatalis (AgMNPV) isoladas em diferentes regiões geográficas
• Beneficiário: Paolo Marinho de Andrade Zanotto
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/03911-7 - Avaliação da interação parasita-hospedeiro do baculovírus AgMNPV em linhagens celulares distintas de Lepidópteras
• Beneficiário: Carla Torres Braconi
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/14537-0 - Análise da diversidade genômica de isolados geográficos do nucleopoliedrovírus de Anticarsia gemmatalis (AgMNPV)
• Beneficiário: Anderson Fernandes de Brito
• Linha de fomento: Bolsas no Brasil - Mestrado