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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Pangenome of the Anticarsia gernmatalis Multiple Nucleopolyhedrovirus (AgMNPV)

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Author(s):
de Brito, Anderson Fernandes [1] ; Braconi, Carla Torres [1] ; Weidmann, Manfred [2] ; Dilcher, Meik [2] ; Pereira Alves, Joao Marcelo [3] ; Gruber, Arthur [3] ; de Andrade Zanotto, Paolo Marinho [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Lab Mol Evolut & Bioinformat, Inst Biomed Sci ICB 2, Dept Microbiol, Sao Paulo, SP - Brazil
[2] Univ Med Ctr, Dept Virol, Gottingen - Germany
[3] Univ Sao Paulo, Dept Parasitol, Inst Biomed Sci ICB 2, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: GENOME BIOLOGY AND EVOLUTION; v. 8, n. 1, p. 94-108, JAN 2016.
Web of Science Citations: 4
Abstract

The alphabaculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) is the world's most successful viral bioinsecticide. Through the 1980s and 1990s, this virus was extensively used for biological control of populations of Anticarsia gemmatalis (Velvetbean caterpillar) in soybean crops. During this period, genetic studies identified several variable loci in the AgMNPV; however, most of them were not characterized at the sequence level. In this study we report a full genome comparison among 17 wild-type isolates of AgMNPV. We found the pangenome of this virus to contain at least 167 hypothetical genes, 151 of which are shared by all genomes. The gene bro-a that might be involved in host specificity and carrying transporter is absent in some genomes, and new hypothetical genes were observed. Among these genes there is a unique rnf12-like gene, probably implicated in ubiquitination. Events of gene fission and fusion are common, as four genes have been observed as single or split open reading frames. Gains and losses of genomic fragments (from 20 to 900 bp) are observed within tandem repeats, such as in eight direct repeats and four homologous regions. Most AgMNPV genes present low nucleotide diversity, and variable genes are mainly located in a locus known to evolve through homologous recombination. The evolution of AgMNPV is mainly driven by small indels, substitutions, gain and loss of nucleotide stretches or entire coding sequences. These variations may cause relevant phenotypic alterations, which probably affect the infectivity of AgMNPV. This work provides novel information on genomic evolution of the AgMNPV in particular and of baculoviruses in general. (AU)

FAPESP's process: 14/03911-7 - Evaluation of host-parasite interaction of the AgMNPV baculovirus with distinct lepidopteran cell lines
Grantee:Carla Torres Braconi
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/14537-0 - Genomic diversity analysis of geographic isolates of nucleopolyhedroviruses Anticarsia gemmatalis (AgMNPV)
Grantee:Anderson Fernandes de Brito
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 11/17120-3 - Genetic diversity analysis of nucleopolyhedrovirus Anticarsia gemmatalis (AgMNPV) populations isolated in different geographic regions
Grantee:Paolo Marinho de Andrade Zanotto
Support Opportunities: Regular Research Grants