Antiviral factors and type I/III interferon expression associated with regulatory factors in the oral epithelial cells from HIV-1-serodiscordant couples

Autor(es)

Cervantes, Cesar A. C. ^[1] ; Oliveira, Luanda M. S. ^[1] ; Manfrere, Kelly C. G. ^[1] ; Lima, Josenilson F. ^[1] ; Pereira, Natalli Z. ^[1] ; Duarte, Alberto J. S. ^[1] ; Sato, Maria N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Trop Med, Dept Dermatol, Lab Dermatol & Immunodeficiencies, LIM 56, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, MAY 11 2016.
• Citações Web of Science: 2

Resumo

Individuals who remain HIV-seronegative despite repeated unprotected exposure to the virus are defined as exposed seronegative (ESN) individuals. Innate and adaptive immunity, as well as genetic factors, provide ESNs with important advantages that allow for low infection susceptibility. The majority of HIV-1-infected individuals undergo antiretroviral therapy, which can decrease the level of HIV-1 exposure in ESNs. We analyzed type I interferon (IFN)-related antiviral and regulatory factors in peripheral blood mononuclear cells (PBMCs) and oral epithelial cells from serodiscordant couples. Our findings revealed that ESNs did not induce the expression of antiviral factors (APOBEC-3G, TRIM5-alpha, SAMDH1, STING, TBk1) or regulatory factors (Trex, Foxo3, Socs3, IL-10) in PBMCs, unlike their HIV-1 infected partners. In contrast, ESNs upregulated APOBEC-3G and type I/III IFNs (IFNs-alpha,-beta/-lambda) in oral mucosal epithelial cells similar to their HIV-infected partners. The serodiscordant groups exhibited an increased expression of type I IFN-induced regulators, such as Trex and Foxo3, in oral epithelial cells. TLR7, TLR8 and TLR9 were expressed in oral epithelial cells of both ESNs and HIV-1-infected subjects. These findings revealed evidence of antiviral factors, type I/III interferon and regulatory factor expression only in the oral mucosal compartment of ESNs, while HIV-1-infected partners systemically and oral mucosal expressed the antiviral profile. (AU)

• Processo FAPESP: 10/15756-5 - Perfil de resposta imune inata induzida por agonistas dos receptores toll-like em indivíduos expostos e não-infectados pelo HIV-1
• Beneficiário: Maria Notomi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/00263-7 - Análise epigenética dos genes envolvidos na resposta de interferon tipo I e perfil funcional de células da imunidade inata e adaptativa em casais sorodiscordantes para o HIV-1
• Beneficiário: Maria Notomi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular