Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

Autor(es)

Bertholim, Luciana ^{[1, 2]} ; Zelanis, Andre ^{[3, 2]} ; Oliveira, Ana K. ^{[1, 2]} ; Serrano, Solange M. T. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil

^[2] Inst Butantan, CeTICS, Lab Especial Toxinol Aplicada, Ctr Toxins Immune Response & Cell Signaling, Av Vital Brasil 1500, BR-05503000 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Amino Acids; v. 48, n. 5, p. 1331-1335, MAY 2016.
• Citações Web of Science: 2

Resumo

The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6' sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1' position, followed by hydrophobic residues in P2'. PICS confirmed existing data on prime site specificity of SVMPs. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 11/16623-1 - Análise proteômica da atividade proteolítica do HF3, uma metaloproteinase do veneno da Bothrops jararaca, no plasma humano e de serpente.
• Beneficiário: Luciana Bertholim Nasciben
• Modalidade de apoio: Bolsas no Brasil - Mestrado