Dietary interesterified fat enriched with palmitic acid induces atherosclerosis by impairing macrophage cholesterol efflux and eliciting inflammation

Autor(es):Mostrar menos -

Afonso, Milessa Silva ^[1] ; Ferrari Lavrador, Maria Silvia ^[1] ; Koike, Marcia Kiyomi ^[2] ; Cintra, Dennys Esper ^[3] ; Ferreira, Fabiana Dias ^[1] ; Nunes, Valeria Sutti ^[1] ; Castilho, Gabriela ^[1] ; Gioielli, Luiz Antonio ^[4] ; Bombo, Renata Paula ^[1] ; Catanozi, Sergio ^[1] ; Caldini, Elia Garcia ^[5] ; Damaceno-Rodrigues, Nilsa Regina ^[5] ; Passarelli, Marisa ^[1] ; Nakandakare, Edna Regina ^[1] ; Lottenberg, Ana Maria ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Sci, Lipids Lab LIM10, Av Dr Arnaldo 455, 3rd Floor, Room 3305, BR-01246903 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Sci, Emergency Care Res Unit Lab LIM51, Av Dr Arnaldo 455, 3rd Floor, Room 3305, BR-01246903 Sao Paulo, SP - Brazil

^[3] Univ Campinas UNICAMP, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeria, SP - Brazil

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Av Dr Arnaldo 455, 3rd Floor, Room 3305, BR-01246903 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Fac Med Sci, Dept Pathol, Lab Cell Biol LIM59, Av Dr Arnaldo 455, 3rd Floor, Room 3305, BR-01246903 Sao Paulo, SP - Brazil

^[6] Albert Einstein Fac Hlth Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 32, p. 91-100, JUN 2016.
• Citações Web of Science: 12

Resumo

Interesterified fats are currently being used to replace trans fatty acids. However, their impact on biological pathways involved in the atherosclerosis development was not investigated. Weaning male LDLr-KO mice were fed for 16 weeks on a high-fat diet (40% energy as fat) containing polyunsaturated (PUFA), TRANS, palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR) or stearic interesterified (STEAR INTER). Plasma lipids, lipoprotein profile, arterial lesion area, macrophage infiltration, collagen content and inflammatory response modulation were determined. Macrophage cholesterol efflux and the arterial expression of cholesterol uptake and efflux receptors were also performed. The interesterification process did not alter plasma lipid concentrations. Although PALM INTER did not increase plasma cholesterol concentration as much as TRANS, the cholesterol enrichment in the LDL particle was similar in both groups. Moreover, PALM INTER induced the highest IL-1 beta, MCP-1 and IL-6 secretion from peritoneal macrophages as compared to others. This inflammatory response elicited by PALM INTER was confirmed in arterial wall, as compared to PALM. These deleterious effects of PALM INTER culminate in higher atherosclerotic lesion, macrophage infiltration and collagen content than PALM, STEAR, STEAR INTER and PUFA. These events can partially be attributed to a macrophage cholesterol accumulation, promoted by apoAl and HDL2-mediated cholesterol efflux impairment and increased Olr-1 and decreased Abca1 and Nr1h3 expressions in the arterial wall. Interesterified fats containing palmitic acid induce atherosclerosis development by promoting cholesterol accumulation in LDL particles and macrophagic cells, activating the inflammatory process in LDLr-KO mice. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/50249-2 - Efeito das gorduras interesterificadas sobre o metabolismo hepatico e adiposo e o desenvolvimento da aterosclerose em camundongos ldlr-ko.
• Beneficiário: Ana Maria Pita Lottenberg
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50443-0 - Efeito de gorduras interesterificadas o desenvolvimento da lesao aterosclerotica em camundongos ldlr knockout.
• Beneficiário: Milessa da Silva Afonso
• Modalidade de apoio: Bolsas no Brasil - Doutorado