Genome-Wide Association Study between Single Nucleotide Polymorphisms and Flight Speed in Nellore Cattle

Autor(es)

Valente, Tiago Silva ^[1] ; Baldi, Fernando ^[1] ; Sant'Anna, Aline Cristina ^[2] ; Albuquerque, Lucia Galvao ^[1] ; Rodrigues Paranhos da Costa, Mateus Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, Dept Zootecnia, Via Acesso Prof Paulo Donato Castellane, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Fed Juiz de Fora, Inst Ciencias Biol, Dept Zool, Rua Jose Lourenco Kelmer, BR-36036900 Juiz de Fora, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 6 JUN 14 2016.
• Citações Web of Science: 2

Resumo

Introduction Cattle temperament is an important factor that affects the profitability of beef cattle enterprises, due to its relationship with productivity traits, animal welfare and labor safety. Temperament is a complex phenotype often assessed by measuring a series of behavioral traits, which result from the effects of multiple environmental and genetic factors, and their interactions. The aims of this study were to perform a genome-wide association study and detect genomic regions, potential candidate genes and their biological mechanisms underlying temperament, measured by flight speed (FS) test in Nellore cattle. Materials and Methods The genome-wide association study (GWAS) was performed using a single-step procedure (ssGBLUP) which combined simultaneously all 16,600 phenotypes from genotyped and non-genotyped animals, full pedigree information of 162,645 animals and 1,384 genotyped animals in one step. The animals were genotyped with High Density Bovine SNP BeadChip which contains 777,962 SNP markers. After quality control (QC) a total of 455,374 SNPs remained. Results Heritability estimated for FS was 0.21 +/- 0.02. Consecutive SNPs explaining 1% or more of the total additive genetic variance were considered as windows associated with FS. Nine candidate regions located on eight different Bos taurus chromosomes (BTA) (1 at 73 Mb, 2 at 65 Mb, 5 at 22 Mb and 119 Mb, 9 at 98 Mb, 11 at 67 Mb, 15 at 16 Mb, 17 at 63 Kb, and 26 at 47 Mb) were identified. The candidate genes identified in these regions were NCKAP5 (BTA2), PARK2 (BTA9), ANTXR1 (BTA11), GUCY1A2 (BTA15), CPE (BTA17) and DOCK1 (BTA26). Among these genes PARK2, GUCY1A2, CPE and DOCK1 are related to dopaminergic system, memory formation, biosynthesis of peptide hormone and neurotransmitter and brain development, respectively. Conclusions Our findings allowed us to identify nine genomic regions (SNP windows) associated with beef cattle temperament, measured by FS test. Within these windows, six promising candidate genes and their biological functions were identified. These results may contribute to a better comprehension into the genetic control of temperament expression in Nellore cattle. (AU)

• Processo FAPESP: 09/16118-5 - Ferramentas genômicas no melhoramento genético de características de importância econômica direta em bovinos da raça Nelore
• Beneficiário: Lucia Galvão de Albuquerque
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/53609-7 - Métodos para avaliação do temperamento de bovinos: estimação de parâmetros genéticos e relações com o desempenho
• Beneficiário: Mateus José Rodrigues Paranhos da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular