Influence of Gender and SNPs in GPX1 Gene on Biomarkers of Selenium Status in Healthy Brazilians

Autor(es)

Donadio, Janaina L. S. ^[1] ; Guerra-Shinohara, Elvira M. ^[2] ; Rogero, Marcelo M. ^[3] ; Cozzolino, Silvia M. F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 8, n. 5 MAY 2016.
• Citações Web of Science: 8

Resumo

Selenium (Se) status varies worldwide as a result of natural variation of Se content in soils, dietary pattern, and the presence of SNPs. Further, Se status in Brazilians and its relationship between genetic variation and Se biomarkers is unknown. This work investigated the association between SNPs in glutathione peroxidase genes and biomarkers of Se status in healthy Brazilians. The study was conducted in 116 healthy adults in Sao Paulo, Brazil. Plasma and erythrocyte Se were measured by HGFAAS. Erythrocyte GPx (eGPx) activity was measured spectrometrically in a biochemical analyzer. Genotypes were determined by real-time PCR using Taqman (R) Assays. eGPx activity was higher in females compared with males. Lower erythrocyte Se concentrations were found in heterozygous GC carriers for GPX1 rs8179169. eGPx activity was higher in females with the common genotypes, except for rs8179169. GC carriers for rs8179169 had lower erythrocyte Se in both genders, and only male carriers of the variant alleles of both rs1050450 and rs1800668 had higher eGPx activity. In conclusion, the genotype for SNPs in GPX1 and gender affected biomarkers of Se status in this pilot study with healthy Brazilians. (AU)

• Processo FAPESP: 09/03047-2 - Associação entre polimorfismos nos genes da glutationa peroxidase e indicadores do estado nutricional relativo ao selênio em população saudável
• Beneficiário: Janaina Lombello Santos Donadio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/11709-5 - Associação entre polimorfismos nos genes da glutationa peroxidase e indicadores do estado nutricional relativo ao selênio em população saudável
• Beneficiário: Silvia Maria Franciscato Cozzolino
• Modalidade de apoio: Auxílio à Pesquisa - Regular