Mammalian cell entry (Mce) protein of Leptospira interrogans binds extracellular matrix components, plasminogen and 2 integrin

Autor(es)

Cosate, Maria Raquel ; Siqueira, Gabriela Hase ; de Souza, Gisele Oliveira ; Vasconcellos, Silvio Arruda ; Nascimento, Ana Lucia T. O.

• Tipo de documento: Artigo Científico
• Fonte: MICROBIOLOGY AND IMMUNOLOGY; v. 60, n. 9, p. 586-598, SEP 2016.
• Citações Web of Science: 3

Resumo

A severe re-emergingzoonosis, leptospirosis, is caused by pathogenic spirochetes of the genus Leptospira. Several studies have identified leptospiral surface proteins with the ability to bind ECM and plasma components, which could mediate adhesion and invasion through the hosts. It has been shown that Mce of pathogenic Leptospira spp. is an RGD (Arg-Gly-Asp)-motif-dependent virulence factor, responsible for infection of cells and animals. In the present article, we decided to further study the repertoire of the Mce activities in leptospiral biological properties. We report that the recombinant Mce is a broad-spectrum ECM-binding protein, capable of interacting with laminin, cellular and plasma fibronectin and collagen IV. Dose-response interaction was observed for all the components, fulfilling ligand-receptor requirements. Mce is a PLG binding protein capable to recruit this component from NHS, generating PLA in the presence of PLG activator. Binding of Mce was also observed with the leukocyte cell receptors L2 {[}(CD11a/CD18)-LFA-1] and M2 {[}(CD11b/CD18)-Mac-1], suggesting the involvement of this protein in the host immune response. Indeed, virulent Leptospira L1-130 was capable of binding both integrins, whereas culture-attenuated M-20 strain only bind to M2 {[}(CD11b/CD18)-Mac-1]. To the best of our knowledge, this is the first work to describe that Mce surface protein could mediate the attachment of Leptospira interrogans to human cell receptors L2(CD11a/CD18) and M2(CD11b/CD18). (AU)

• Processo FAPESP: 12/51165-7 - Avaliacao e caracterizacao de lipoproteinasde leptospira interrogans para o entendimentoda interacao patogeno-hospedeiro.
• Beneficiário: Maria Raquel Venturim Cosate
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50981-0 - Busca de proteínas de superfície nas sequências do genoma da Leptospira interrogans: caracterização funcional e imunológica para o entendimento de mecanismos envolvidos na patogênese de bactéria
• Beneficiário: Ana Lucia Tabet Oller do Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/23913-9 - Identificação e caracterização de proteínas de Leptospira interrogans envolvidas nas interações patógeno - hospedeiro
• Beneficiário: Ana Lucia Tabet Oller do Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/03792-8 - "Avaliação da evasão do sistema imunológico por cepas patogênicas de Leptospira interrogans a nível da via terminal do sistema complemento"
• Beneficiário: Gabriela Hase Siqueira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado