A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

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Phan, Tung G. ; Dreno, Brigitte ; da Costa, Antonio Charlys ; Li, Linlin ; Orlandi, Patricia ; Deng, Xutao ; Kapusinszky, Beatrix ; Siqueira, Juliana ; Knol, Anne-Chantal ; Halary, Franck ; Dantal, Jacques ; Alexander, Kathleen A. ; Pesavento, Patricia A. ; Delwart, Eric

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY; v. 496, p. 299-305, SEP 2016.
• Citações Web of Science: 16

Resumo

We genetically characterized seven nearly complete genomes in the protoparvovirus genus from the feces of children with diarrhea. The viruses, provisionally named cutaviruses (CutaV), varied by 1-6% nucleotides and shared similar to 76% and similar to 82% amino acid identity with the NS1 and VP1 of human bufaviruses, their closest relatives. Using PCR, cutavirus DNA was found in 1.6% (4/245) and 1% (1/100) of diarrhea samples from Brazil and Botswana respectively. In silico analysis of pre-existing metagenomics datasets then revealed closely related parvovirus genomes in skin biopsies from patients with epidermotropic cutaneous T-cell lymphoma (CTCL or mycosis fungoides). PCR of skin biopsies yielded cutavirus DNA in 4/17 CTCL, 0/10 skin carcinoma, and 0/21 normal or noncancerous skin biopsies. In situ hybridization of CTCL skin biopsies detected viral genome within rare individual cells in regions of neoplastic infiltrations. The influence of cutavirus infection on human enteric functions and possible oncolytic role in CTCL progression remain to be determined. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 14/05211-2 - Metagenômica viral em plasma de doadores de sangue em dois bancos de sangue brasileiro
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 12/03417-7 - Caracterização do vírus da dengue pela análise do genoma completo viral em amostras de doadores e receptores de sangue nos estados de Pernambuco e Rio de Janeiro
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto