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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

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Phan, Tung G. ; Dreno, Brigitte ; da Costa, Antonio Charlys ; Li, Linlin ; Orlandi, Patricia ; Deng, Xutao ; Kapusinszky, Beatrix ; Siqueira, Juliana ; Knol, Anne-Chantal ; Halary, Franck ; Dantal, Jacques ; Alexander, Kathleen A. ; Pesavento, Patricia A. ; Delwart, Eric
Total Authors: 14
Document type: Journal article
Source: VIROLOGY; v. 496, p. 299-305, SEP 2016.
Web of Science Citations: 16
Abstract

We genetically characterized seven nearly complete genomes in the protoparvovirus genus from the feces of children with diarrhea. The viruses, provisionally named cutaviruses (CutaV), varied by 1-6% nucleotides and shared similar to 76% and similar to 82% amino acid identity with the NS1 and VP1 of human bufaviruses, their closest relatives. Using PCR, cutavirus DNA was found in 1.6% (4/245) and 1% (1/100) of diarrhea samples from Brazil and Botswana respectively. In silico analysis of pre-existing metagenomics datasets then revealed closely related parvovirus genomes in skin biopsies from patients with epidermotropic cutaneous T-cell lymphoma (CTCL or mycosis fungoides). PCR of skin biopsies yielded cutavirus DNA in 4/17 CTCL, 0/10 skin carcinoma, and 0/21 normal or noncancerous skin biopsies. In situ hybridization of CTCL skin biopsies detected viral genome within rare individual cells in regions of neoplastic infiltrations. The influence of cutavirus infection on human enteric functions and possible oncolytic role in CTCL progression remain to be determined. (C) 2016 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 14/05211-2 - Viral metagenomics in human plasma of blood donors in two blood centers in Brazil
Grantee:Antonio Charlys da Costa
Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
FAPESP's process: 12/03417-7 - Complete genomes for dengue virus: viral genetic diversity among seropositive first-time blood donors and recipients in two blood centres in Brazil
Grantee:Antonio Charlys da Costa
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)