Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice

Autor(es)

Leite, Nayara Carvalho ; de Paula, Flavia ; Borck, Patricia Cristine ; Vettorazzi, Jean Franciesco ; Souto Branco, Renato Chaves ; Lubaczeuski, Camila ; Boschero, Antonio Carlos ; Zoppi, Claudio Cesar ; Carneiro, Everardo Magalhaes

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, SEP 16 2016.
• Citações Web of Science: 6

Resumo

Pancreatic beta cell (beta) dysfunction is an outcome of malnutrition. We assessed the role of the amplifying pathway (AMP PATH) in beta cells in malnourished obese mice. C57Bl-6 mice were fed a control (C) or a low-protein diet (R). The groups were then fed a high-fat diet (CH and RH). AMP PATH contribution to insulin secretion was assessed upon incubating islets with diazoxide and KCl. CH and RH displayed increased glucose intolerance, insulin resistance and glucose-stimulated insulin secretion. Only RH showed a higher contribution of the AMP PATH. The mitochondrial membrane potential of RH was decreased, and ATP flux was unaltered. In RH islets, glutamate dehydrogenase (GDH) protein content and activity increased, and the AMP PATH contribution was reestablished when GDH was blunted. Thus, protein malnutrition induces mitochondrial dysfunction in beta cells, leading to an increased contribution of the AMP PATH to insulin secretion through the enhancement of GDH content and activity. (AU)

• Processo FAPESP: 11/19536-2 - Controle metabólico do processo de secreção de insulina em ilhotas de Langerhans de camundongos submetidos à desnutrição protéica e obesidade experimental
• Beneficiário: Everardo Magalhães Carneiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
• Beneficiário: Licio Augusto Velloso
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 11/09012-6 - Mecanismos moleculares envolvidos na disfunção e morte de células beta pancreáticas no Diabetes Mellitus: estratégias para a inibição desses processos e para a recuperação da massa insular em diferentes modelos celulares e animais
• Beneficiário: Antonio Carlos Boschiero
• Modalidade de apoio: Auxílio à Pesquisa - Temático