Evidence of bottleneck effect on hepatitis C virus transmission between a couple under interferon based therapy

Autor(es)

da Silva, Rafael Alves ; Vicente Guedes de Carvalho, Isabel Maria ; Adum de Matos, Renata Prandini ; Tomonari Yamasaki, Lilian Hiromi ; Bittar, Cintia ; Rahal, Paula ; Gomes Jardim, Ana Carolina

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 47, p. 87-93, JAN 2017.
• Citações Web of Science: 1

Resumo

Issues on the correlation of viral genetic diversity and treatment response to the hepatitis C infection remain uncertain. The bottleneck effect dictates the characteristics of the viral population that will establish the infection in a new host and is related to how the immune system and treatment will be effective against the virus. Here we evaluated the phylogenetic characteristics of quasispecies population and the treatment response pattern of a HCV infected couple. We also analyzed whether the viral population of these patients indicated that they were exposed to the same source for primer infection. This study included two patients (P10 and P11) HCV genotype 1b infected. The couple presented horizontal transmission. Viral RNA was isolated from serum samples collected before, during and after treatment, at specific time points. The HCV NS5A gene sequence was amplified, cloned and sequenced. Genetic and evolutionary analyses were performed to compare the quasispecies population of these two patients and local control patients. Genetic distance and diversity were calculated. Phylogenetic analyses were performed by using maximum likelihood and Bayesian methodologies. The analysis of the baseline samples showed that the genetic distance of the viral populations of patients P10 and P11 was significantly lower than when these patients and the control group based on sequences from local patients were analyzed, supporting the horizontal transmission hypothesis. Phylogenetic analysis with sequences from all the time point samples also demonstrated two patterns of evolution depending on the treatment response. The Bayesian analysis showed that one isolate corresponding to the baseline sample of P10 was grouped into the P11 clade, suggesting a way of infection and a bottleneck effect. Our data suggests that the patient P11 viral population may be originated from variants from P10 patient and consequently showing that clinical differences between treatment responses can emerge from the bottleneck effect on viral populations. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/18168-2 - Caracterização molecular dò Vírus da Hepatite C Èm portadores dè doença renal crônica Èm hemodiálise
• Beneficiário: Rafael Alves da Silva
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/52073-0 - Análise das quasispecies da região genômica NS5A do Vírus da Hepatite C (HCV) genótipo 1, e da carga viral, em pacientes respondedores, não respondedores e respondedores ao final do tratamento
• Beneficiário: Ana Carolina Gomes Jardim
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/51165-1 - Estudo da evolução dás quasiespécies da região NS5A dè HCV genótipo 3ª
• Beneficiário: Cintia Bittar Oliva
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/06156-4 - Investigação da dinâmica de quasispecies em casais infectados com HCV
• Beneficiário: Renata Prandini Adum de Matos
• Linha de fomento: Bolsas no Brasil - Iniciação Científica