The effect of two drug delivery systems in ropivacaine cytotoxicity and cytokine release by human keratinocytes and fibroblasts

Autor(es)

Nunes Ferreira, Luiz Eduardo ; Muniz, Bruno Vilela ; Burga-Sanchez, Jonny ; Volpato, Maria Cristina ; de Paula, Eneida ; Ribeiro Rosa, Edvaldo Antonio ; Groppo, Francisco Carlos

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmacy and Pharmacology; v. 69, n. 2, p. 161-171, FEB 2017.
• Citações Web of Science: 5

Resumo

ObjectivesModified drug delivery systems have been developed to improve pharmacological properties of local anaesthetics. However, the inflammatory potential of these formulations was not investigated. This study compared the in-vitro effects of ropivacaine (ropi) in plain, liposomal (MLV) or 2-hydroxypropyl--cyclodextrin (HP--CD) formulations on cell viability, apoptosis and cytokine (IL-1, TNF-, IL-6 and IL-10) release. MethodsHuman immortalized keratinocytes (HaCaT) and human immortalized gingival fibroblasts (HGF) were exposed to 1-100m ropi concentrations. The cell viability was measured by XTT and LIVE/DEAD assay. Apoptosis was performed by flow cytometry, and cytokine release was measured by ELISA assay. Key findingsHuman immortalized keratinocyte viability was reduced by ropi and both drug delivery systems. However, none of the formulations induced apoptosis. Results showed a differential regulation of IL-1 TNF-, IL-6 and IL-10 by HaCaT and HGF. Ropi-HP--CD increased twofold the IL-6 release by HGF in comparison with the control, while 100m ropi-MLV led to an increased release of all pro-inflammatory cytokines by HGF. ConclusionThe loss in cell viability was not related to cellular apoptosis. Ropi complexed with HP--CD showed a similar cytokine release pattern when compared to the plain formulation. Thus, the HP--CD form was a better drug carrier than the MLV form for ropivacaine drug delivery. (AU)

• Processo FAPESP: 12/07310-2 - Efeitos modulatórios de anestésicos locais associados a carreadores sobre a produção de mediadores inflamatórios e viabilidade celular em células epiteliais orais e fibroblastos gengivais humanos
• Beneficiário: Francisco Carlos Groppo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/12666-8 - Efeitos dos anestésicos locais associados a carreadores na modulação de mediadores inflamatórios, viabilidade celular e indução da apoptose
• Beneficiário: Luiz Eduardo Nunes Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto