Effective characterization of Salmonella Enteritidis by most probable number (MPN) followed by multiplex polymerase chain reaction (PCR) methods

Autor(es)

Zappelini, Lincohn ; Martone-Rocha, Solange ; Dropa, Milena ; Matte, Maria Helena ; Tiba, Monique Ribeiro ; Breternitz, Bruna Suellen ; Pepe Razzolini, Maria Tereza

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 24, n. 5, p. 4828-4834, FEB 2017.
• Citações Web of Science: 1

Resumo

Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in raw sewage. A total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex PCR methods. From those, 123 isolates were selected based on PCR banding patternidentical or similar to Salmonella Enteritidis and Salmonella Typhimurium-and submitted to conventional serotyping. Results showed that both PCR assays correctly serotyped Salmonella Enteritidis, however, they presented ambiguous results for Salmonella Typhimurium identification. These data highlight that MPN and multiplex PCR can be useful methods to describe microbial quality in raw sewage and suggest two new PCR patterns for Salmonella Enteritidis identification. (AU)

• Processo FAPESP: 10/50797-4 - Modelo de estudo para avaliar o impacto de patógenos entéricos em mananciais de abastecimento público
• Beneficiário: Maria Helena Matte
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE