| Full text | |
| Author(s): |
Zappelini, Lincohn
;
Martone-Rocha, Solange
;
Dropa, Milena
;
Matte, Maria Helena
;
Tiba, Monique Ribeiro
;
Breternitz, Bruna Suellen
;
Pepe Razzolini, Maria Tereza
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Environmental Science and Pollution Research; v. 24, n. 5, p. 4828-4834, FEB 2017. |
| Web of Science Citations: | 1 |
| Abstract | |
Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in raw sewage. A total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex PCR methods. From those, 123 isolates were selected based on PCR banding patternidentical or similar to Salmonella Enteritidis and Salmonella Typhimurium-and submitted to conventional serotyping. Results showed that both PCR assays correctly serotyped Salmonella Enteritidis, however, they presented ambiguous results for Salmonella Typhimurium identification. These data highlight that MPN and multiplex PCR can be useful methods to describe microbial quality in raw sewage and suggest two new PCR patterns for Salmonella Enteritidis identification. (AU) | |
| FAPESP's process: | 10/50797-4 - Model study to assess the impact of enteric pathogens in public water supply sources |
| Grantee: | Maria Helena Matte |
| Support Opportunities: | Research Grants - Research Partnership for Technological Innovation - PITE |