Heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview

Autor(es)

Ferreira, Jr., Rui Seabra ; de Barros, Luciana Curtolo ; Fernandes Abbade, Luciana Patircia ; Catharino Sartori Barraviera, Silvia Regina ; Cavariani Silvares, Maria Regina ; de Pontes, Leticia Gomes ; dos Santos, Lucilene Delazari ; Barraviera, Benedito

• Tipo de documento: Artigo de Revisão
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 23, n. 1 APR 4 2017.
• Citações Web of Science: 19

Resumo

Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its preclinical applications aiming at repairing nervous system traumas and regenerating bone marrow. Finally, we present an innovative safety trial I/II that found the product to be a safe and clinically promising candidate for treating chronic venous ulcers. A multicenter clinical trial, phase II/III, with a larger number of participants will be performed to prove the efficacy of an innovative biopharmaceutical product derived from animal venom. (AU)

• Processo FAPESP: 14/13299-7 - Identificação de biomarcadores moleculares prognósticos e diagnósticos de cicatrização no exsudato inflamatório de úlceras venosas crônicas antes e após o tratamento com selante de fibrina
• Beneficiário: Lucilene Delazari dos Santos
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/53846-9 - EMU: aquisição de cromatógrafo líquido de alta eficiência (CLAE) preparativo para purificação e isolamento de serinoproteinases para produção do selante de fibrina
• Beneficiário: Benedito Barraviera
• Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 12/08101-8 - Imageamento in vivo da regeneração nervosa após reimplante de raízes motoras com selante de fibrina fluo-marcado associado com células tronco mesenquimais
• Beneficiário: Rui Seabra Ferreira Junior
• Linha de fomento: Auxílio à Pesquisa - Regular