Early suppression of adipocyte lipid turnover induces immunometabolic modulation in cancer cachexia syndrome

Autor(es)

Henriques, Felipe Santos ; Laurato Sertie, Rogerio Antonio ; Franco, Felipe Oliveira ; Knobl, Pamela ; Neves, Rodrigo Xavier ; Andreotti, Sandra ; Lima, Fabio Bessa ; Guilherme, Adilson ; Seelaender, Marilia ; Batista, Jr., Miguel Luiz

• Tipo de documento: Artigo Científico
• Fonte: FASEB JOURNAL; v. 31, n. 5, p. 1976-1986, MAY 2017.
• Citações Web of Science: 5

Resumo

Cancer cachexia is a multifactorial syndrome characterized by body weight loss, atrophy of adipose tissue (AT) and systemic inflammation. However, there is limited information regarding the mechanisms of immunometabolic response in AT from cancer cachexia. Male Wistar rats were inoculated with 2 3 107 of Walker 256 tumor cells {[}tumor bearing (TB) rats]. The mesenteric AT (MeAT) was collected on d 0, 4, 7 (early stage), and 14 (cachexia stage) after tumor cell injection. Surgical biopsies for MeAT were obtained from patients who had gastrointestinal cancer with cachexia. Lipolysis showed an early decrease in glycerol release in TB d 4 (TB4) rats in relation to the control, followed by a 6-fold increase in TB14 rats, whereas de novo lipogenesis was markedly lower in the incorporation of glucose into fatty acids in TB14 rats during the development of cachexia. CD11b and CD68 were positive in TB7 and TB14 rats, respectively. In addition, we found cachexia stage results similar to those of animals in MeAT from patients: an increased presence of CD68(+), iNOS2(+), TNF alpha(+), and HSL+ cells. In summary, translational analysis of MeAT from patients and an animal model of cancer cachexia enabled us to identify early disruption in Adl turnover and subsequent inflammatory response during the development of cancer cachexia.-Henriques, F. S., Serti ` e, R. A. L., Franco, F. O., Knobl, P., Neves, R. X., Andreotti, S., Lima, F. B., Guilherme, A., Seelaender, M., Batista, M. L., Jr. Early suppression of adipocyte lipid turnover induces immunometabolic modulation in cancer cachexia syndrome. (AU)

• Processo FAPESP: 15/19259-0 - Repercussões do remodelamento do tecido adiposo durante a síndrome de caquexia em pacientes com câncer gastrointestinal: potencial envolvimento do receptor TLR4 na modulação do browning no TAB
• Beneficiário: Miguel Luiz Batista Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51078-1 - Bases moleculares da caquexia: adipogênese e remodelagem da matriz extracelular do tecido adiposo branco de pacientes com câncer gastrointestinal
• Beneficiário: Miguel Luiz Batista Junior
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores