Palmitoleic acid reduces the inflammation in LPS-stimulated macrophages by inhibition of NF kappa B, independently of PPARs

Autor(es)

Souza, Camila O. ; Teixeira, Alexandre A. S. ; Biondo, Luana A. ; Silveira, Loreana S. ; Calder, Philip C. ; Rosa Neto, Jose C.

• Tipo de documento: Artigo Científico
• Fonte: Clinical and Experimental Pharmacology and Physiology; v. 44, n. 5, p. 566-575, MAY 2017.
• Citações Web of Science: 14

Resumo

Palmitoleic acid (PM, 16:1n-7) has anti-inflammatory properties that could be linked to higher expression of PPAR, an inhibitor of NFB. Macrophages play a major role in the pathogenesis of chronic inflammation, however, the effects of PM on macrophages are underexplored. Thus, we aimed to investigate the effects of PM in activated macrophages as well the role of PPAR. Primary macrophages were isolated from C57BL/6 wild type (WT) and PPAR knockout (KO) mice, cultured under standard conditions and exposed to lipopolysaccharides LPS (2.5g/ml) and PM 600mol/L conjugated with albumin for 24hours. The stimulation with LPS increased the production of interleukin (IL)-6 and IL-1 while PM decreased the production of IL-6 in WT macrophages. In KO macrophages, LPS increased the production of tumour necrosis factor (TNF)- and IL-6 and PM decreased the production of TNF. The expression of inflammatory markers such NFB and IL1 were increased by LPS and decreased by PM in both WT and KO macrophages. PM reduced the expression of MyD88 and caspase-1 in KO macrophages, and the expression of TLR4 and HIF-1 in both WT and KO macrophages, although LPS had no effect. CD86, an inflammatory macrophage marker, was reduced by PM independently of genotype. PM increased PPAR and reduced PPAR gene expression in macrophages of both genotypes, and increased ACOX-1 expression in KO macrophages. In conclusion, PM promotes anti-inflammatory effects in macrophages exposed to LPS through inhibition of inflammasome pathway, which was independent of PPAR, PPAR? and AMPK, thus the molecular mechanisms of anti-inflammatory response caused by PM is still unclear. (AU)

• Processo FAPESP: 16/01409-8 - Caracterização do efeito antiinflamatório da suplementação com ácido palmitoleico na inflamação hepática; o papel dos ppars.
• Beneficiário: José Cesar Rosa Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/04765-1 - Efeitos da suplementação com ácido palmitoléico nas alterações imuno-metabólicas hepáticas induzidas por dieta hiperlipídica em camundongos PPAR± -/-
• Beneficiário: Camila Oliveira de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado