DEPLETION OF ROSTRAL VENTROLATERAL MEDULLARY CATECHOLAMINERGIC NEURONS IMPAIRS THE HYPDXIC VENTILATORY RESPONSE IN CONSCIOUS RATS

Autor(es)

Malheiros-Lima, Milene R. ; Takakura, Ana C. ; Moreira, Thiago S.

• Tipo de documento: Artigo Científico
• Fonte: Neuroscience; v. 351, p. 1-14, MAY 20 2017.
• Citações Web of Science: 11

Resumo

The stimuli that commonly activate the cate-cholaminergic C1 neurons (nociception, hypotension, and hypoxia) also increase breathing. Pharmacogenetic evidence suggests that catecholaminergic neurons regulate breathing. Therefore, we evaluated whether the loss of C1 cells affects cardiorespiratory control during resting, hypoxic (8% O-2) and hypercapnic (7% CO2) conditions. A bilateral injection of the immunotoxin anti-dopamine beta-hydroxylase-saporin (anti-D beta H-SAP; 2.4 ng/100 nl) or saline was performed in adult male Wistar rats (270-300 g, N = 5-8/group). Histology revealed a 60-75% loss of C1 neurons in anti-D beta H-SAP-treated rats, but no significant changes or C1 cell loss was observed in sham-treated rats or those with off-target injection sites. Bilateral depletion of C1 neurons did not alter cardiorespiratory variables during rest and hypercapnia (7% CO2), but it did affect the response to hypoxia. Specifically, the increase in ventilation, the number of sighs, and the tachycardia were reduced, but unexpectedly, the mean arterial pressure increased during hypoxia (8% 02). The present study indicates that C1 neurons contribute to cardiorespiratory control during hypoxia rather than at rest or during hypercapnia. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 15/23376-1 - Núcleo retrotrapezóide, quimiossensibilidade central e automaticidade respiratória
• Beneficiário: Thiago dos Santos Moreira
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/07698-6 - Envolvimento dos neurônios catecolaminérgicos do bulbo ventrolateral rostral no controle da atividade respiratória
• Beneficiário: Milene Rodrigues Malheiros Lima
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/22406-1 - Alterações respiratórias anatomofuncionais observadas em um modelo experimental da Doença de Parkinson
• Beneficiário: Ana Carolina Thomaz Takakura
• Linha de fomento: Auxílio à Pesquisa - Regular