Exomic variants of an elderly cohort of Brazilians in the ABraOM database

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Naslavsky, Michel Satya ; Yamamoto, Guilherme Lopes ; de Almeida, Tatiana Ferreira ; Ezquina, Suzana A. M. ; Sunaga, Daniele Yumi ; Pho, Nam ; Bozoklian, Daniel ; Milkewitz Sandberg, Tatiana Orli ; Brito, Luciano Abreu ; Lazar, Monize ; Bernardo, Danilo Vicensotto ; Amaro, Jr., Edson ; Duarte, Yeda A. O. ; Lebrao, Maria Lucia ; Passos-Bueno, Maria Rita ; Zatz, Mayana

• Tipo de documento: Artigo Científico
• Fonte: Human mutation; v. 38, n. 7, p. 751-763, JUL 2017.
• Citações Web of Science: 45

Resumo

Brazilians are highly admixed with ancestry from Europe, Africa, America, and Asia and yet still underrepresented in genomic databanks. We hereby present a collection of exomic variants from 609 elderly Brazilians in a census-based cohort (SABE609) with comprehensive phenotyping. Variants were deposited in ABraOM (Online Archive of Brazilian Mutations), a Web-based public database. Population representative phenotype and genotype repositories are essential for variant interpretation through allele frequency filtering; since elderly individuals are less likely to harbor pathogenic mutations for early- and adult-onset diseases, such variant databases are of great interest. Among the over 2.3 million variants from the present cohort, 1,282,008 were high-confidence calls. Importantly, 207,621 variants were absent from major public databases. We found 9,791 potential loss-of-function variants with about 300 mutations per individual. Pathogenic variants on clinically relevant genes (ACMG) were observed in 1.15% of the individuals and were correlated with clinical phenotype. We conducted incidence estimation for prevalent recessive disorders based upon heterozygous frequency and concluded that it relies on appropriate pathogenicity assertion. These observations illustrate the relevance of collecting demographic data from diverse, poorly characterized populations. Census-based datasets of aged individuals with comprehensive phenotyping are an invaluable resource toward the improved understanding of variant pathogenicity. (AU)

• Processo FAPESP: 11/17428-8 - Componentes genéticos da dominância manual e lateralidade cerebral em idosos saudáveis
• Beneficiário: Michel Satya Naslavsky
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 98/14254-2 - Centro de Estudos do Genoma Humano
• Beneficiário: Mayana Zatz
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/08028-1 - CEGH-CEL - Centro de Estudos do Genoma Humano e de Células-Tronco
• Beneficiário: Mayana Zatz
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 08/57899-7 - Células tronco em doenças genéticas humanas - CETGEN
• Beneficiário: Mayana Zatz
• Modalidade de apoio: Auxílio à Pesquisa - Temático