| Texto completo | |
| Autor(es): |
Rodrigues, Fernanda Luciano
;
Fais, Rafael S.
;
Pereira, Marlia G. A. G.
;
Garcia-Cairasco, Norberto
;
Tostes, Rita C.
;
Carneiro, Fernando S.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | UROLOGY; v. 106, AUG 2017. |
| Citações Web of Science: | 0 |
| Resumo | |
OBJECTIVE To test the hypothesis that naive Wistar audiogenic rats (WARs) display erectile dysfunction (ED), which is associated with increased sympathetic-mediated contractile tone and decreased nitric oxide-mediated relaxation responses of the cavernous tissue. METHODS Changes in the ratio of the maximal intracavernosal pressure-mean arterial pressure after the electrical stimulation of the right major pelvic ganglion were determined in vivo. Cavernosal contractility was induced by electrical field stimulation and phenylephrine. In addition, nonadrenergic-noncholinergic (NANC)-induced relaxation was determined. Rho-kinase (ROCK) pathway proteins, neuronal nitric oxide synthase (nNOS) protein expression, and endothelial nitric oxide synthase (eNOS) and extracellular signal-regulated kinase 1/2 activities were determined by Western blot. RESULTS WARs display a significant decrease in maximal intracavernosal pressure-mean arterial pressure responses suggesting ED in this strain. Sympathetic-mediated contractile responses were increased in WARs and contractile responses to phenylephrine were not changed. The increased sympathetic-mediated contractile responses were not associated with changes in the ROCK pathway. On the other hand, NANC-mediated relaxation responses were significantly reduced in WARs. This functional response was accompanied by decreased nNOS and total eNOS protein expressions, augmented phosphorylated eNOS, and decreased extracellular signal-regulated kinase 1/2 phosphorylation levels. CONCLUSION Our data have demonstrated that naive WARs display ED in vivo that is associated with increased sympathetic-mediated contractile responses and decreased NANC-mediated relaxation responses. The increase in contractile responses is independent of the ROCK pathway, and the changes in relaxation responses are associated with a decrease in nNOS protein expression, which may activate compensatory mechanisms in the cavernous tissue. (C) 2017 Elsevier Inc. (AU) | |
| Processo FAPESP: | 10/17362-4 - Mecanismos da disfunção erétil na insuficiência cardíaca |
| Beneficiário: | Fernando Silva Carneiro |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 15/21976-1 - Controle autonômico cardiovascular em camundongos knockout para receptores toll-like 9 com hipertensão induzida por angiotensina II. |
| Beneficiário: | Fernanda Luciano Rodrigues |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 07/50261-4 - Neurociências, epilepsia e arte: caracterização interdisciplinar da cepa WAR e modelos de epilepsia correlacionados: as oficinas Da Vinci, divulgação científica e quebras de estigmas em epilepsia |
| Beneficiário: | Norberto Garcia Cairasco |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos |
| Beneficiário: | João Pereira Leite |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa CINAPCE - Temático |
| Processo FAPESP: | 16/11988-5 - Contribuição do NLRP3 para o efeito pró-inflamatório da ET-1 em células de músculo liso do corpo cavernoso: relevância na função erétil. |
| Beneficiário: | Fernando Silva Carneiro |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |