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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Erectile Dysfunction in Wistar Audiogenic Rats Is Associated With Increased Cavernosal Contraction and Decreased Neuronal Nitric Oxide Synthase Protein Expression

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Author(s):
Rodrigues, Fernanda Luciano ; Fais, Rafael S. ; Pereira, Marlia G. A. G. ; Garcia-Cairasco, Norberto ; Tostes, Rita C. ; Carneiro, Fernando S.
Total Authors: 6
Document type: Journal article
Source: UROLOGY; v. 106, AUG 2017.
Web of Science Citations: 0
Abstract

OBJECTIVE To test the hypothesis that naive Wistar audiogenic rats (WARs) display erectile dysfunction (ED), which is associated with increased sympathetic-mediated contractile tone and decreased nitric oxide-mediated relaxation responses of the cavernous tissue. METHODS Changes in the ratio of the maximal intracavernosal pressure-mean arterial pressure after the electrical stimulation of the right major pelvic ganglion were determined in vivo. Cavernosal contractility was induced by electrical field stimulation and phenylephrine. In addition, nonadrenergic-noncholinergic (NANC)-induced relaxation was determined. Rho-kinase (ROCK) pathway proteins, neuronal nitric oxide synthase (nNOS) protein expression, and endothelial nitric oxide synthase (eNOS) and extracellular signal-regulated kinase 1/2 activities were determined by Western blot. RESULTS WARs display a significant decrease in maximal intracavernosal pressure-mean arterial pressure responses suggesting ED in this strain. Sympathetic-mediated contractile responses were increased in WARs and contractile responses to phenylephrine were not changed. The increased sympathetic-mediated contractile responses were not associated with changes in the ROCK pathway. On the other hand, NANC-mediated relaxation responses were significantly reduced in WARs. This functional response was accompanied by decreased nNOS and total eNOS protein expressions, augmented phosphorylated eNOS, and decreased extracellular signal-regulated kinase 1/2 phosphorylation levels. CONCLUSION Our data have demonstrated that naive WARs display ED in vivo that is associated with increased sympathetic-mediated contractile responses and decreased NANC-mediated relaxation responses. The increase in contractile responses is independent of the ROCK pathway, and the changes in relaxation responses are associated with a decrease in nNOS protein expression, which may activate compensatory mechanisms in the cavernous tissue. (C) 2017 Elsevier Inc. (AU)

FAPESP's process: 10/17362-4 - Mechanisms of erectile dysfunction on heart failure
Grantee:Fernando Silva Carneiro
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/21976-1 - Cardiovascular autonomic control in toll-like receptor 9 knockout mice with hypertension induced by angiotensin II.
Grantee:Fernanda Luciano Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 05/56447-7 - Research through images from high field magnetic resonance aimed at studies in humans
Grantee:João Pereira Leite
Support Opportunities: Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants
FAPESP's process: 16/11988-5 - NLRP3 contribution to the pro-inflammatory effect of ET-1 in corpora cavernosa smooth muscle cells: relevance in erectile function.
Grantee:Fernando Silva Carneiro
Support Opportunities: Regular Research Grants