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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Crystal Structure and Regulation of the Citrus Pol III Repressor MAF1 by Auxin and Phosphorylation

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Soprano, Adriana Santos ; de Giuseppe, Priscila Oliveira ; Shimo, Hugo Massayoshi ; Lima, Tatiani Brenelli ; Heleno Batista, Fernanda Aparecida ; Righetto, Germanna Lima ; de Carvalho Pereira, Jose Geraldo ; Granato, Daniela Campos ; Ziem Nascimento, Andrey Fabricio ; Gozzo, Fabio Cesar ; Lopes de Oliveira, Paulo Sergio ; Migliorini Figueira, Ana Carolina ; Costa Smetana, Juliana Helena ; Paes Leme, Adriana Franco ; Murakami, Mario Tyago ; Benedetti, Celso Eduardo
Número total de Autores: 16
Tipo de documento: Artigo Científico
Fonte: Structure; v. 25, n. 9, p. 1360+, SEP 5 2017.
Citações Web of Science: 9
Resumo

MAF1 is the main RNA polymerase (Pol) III repressor that controls cell growth in eukaryotes. The Citrus ortholog, CsMAF1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. We solved the crystal structure of the globular core of CsMAF1, which reveals additional structural elements compared with the previously available structure of hMAF1, and explored the dynamics of its flexible regions not present in the structure. CsMAF1 accumulated in the nucleolus upon leaf excision, and this translocation was inhibited by auxin and by mutation of the PKA phosphorylation site, S45, to aspartate. Additionally, mTOR phosphorylated recombinant CsMAF1 and the mTOR inhibitor AZD8055 blocked canker formation in normal but not CsMAF1-silenced plants. These results indicate that the role of TOR on cell growth induced by Xanthomonas citri depends on CsMAF1 and that auxin controls CsMAF1 interaction with Pol III in citrus. (AU)

Processo FAPESP: 11/20468-1 - Modelos biológicos de interação planta-patógeno para o entendimento de mecanismos de patogenicidade e adaptação de fitobactérias, respostas de defesa e desenvolvimento de doença em citros
Beneficiário:Celso Eduardo Benedetti
Linha de fomento: Auxílio à Pesquisa - Temático