Involvement of Dihydrolipoyl Dehydrogenase in the Phagocytosis and Killing of Paracoccidioides brasiliensis by Macrophages

Autor(es)

Landgraf, Taise N. ; Costa, Marcelo V. ; Oliveira, Aline F. ; Ribeiro, Wander C. ; Panunto-Castelo, Ademilson ; Fernandes, Fabricio F.

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 8, SEP 20 2017.
• Citações Web of Science: 0

Resumo

Paracoccidioides brasiliensis and Paracoccidioides lutzii are fungi causing paracoccidioidomycosis (PCM), an autochthonous systemic mycosis found in Latin America. These microorganisms contain a multitude of molecules that may be associated with the complex interaction of the fungus with the host. Here, we identify the enzyme dihydrolipoyl dehydrogenase (DLD) as an exoantigen from P. brasiliensis (Pb18\_Dld) by mass spectrometry. Interestingly, the DLD gene expression in yeast form showed higher expression levels than those in mycelial form and transitional phases. Pb18\_Dld gene was cloned, and the recombinant protein (rPb18\_Dld) was expressed and purified for subsequent studies and production of antibodies. Immunogold labeling and transmission electron microscopy revealed that the Pb18\_Dld is also localized in mitochondria and cytoplasm of P. brasiliensis. Moreover, when macrophages were stimulated with rPb18Dld, there was an increase in the phagocytic and microbicidal activity of these cells, as compared with non-stimulated cells. These findings suggest that Pb18\_Dld can be involved in the pathogen-host interaction, opening possibilities for studies of this protein in PCM. (AU)

• Processo FAPESP: 16/00629-4 - Caracterização de domínios enzimático e de lectina e os efeitos sobre receptores de células da imunidade inata
• Beneficiário: Fabrício Freitas Fernandes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/08552-0 - Identificação de antígenos de Paracoccidioides brasiliensis com potencial imunoterapêutico para o tratamento de paracoccidioidomicose.
• Beneficiário: Taise Natali Landgraf
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/12278-3 - Rastreamento de antígenos de Paracoccidioides brasiliensis com potencial imunoterapêutico na paracoccidioidomicose
• Beneficiário: Ademilson Panunto-Castelo
• Modalidade de apoio: Auxílio à Pesquisa - Regular