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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A validated HPLC-UV method for the analysis of galloylquinic acid derivatives and flavonoids in Copaifera langsdorffii leaves

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Autor(es):
da Silva Motta, Erick Vicente [1] ; da Costa, Juliana de Carvalho [1] ; Bastos, Jairo Kenupp [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1061, p. 240-247, SEP 1 2017.
Citações Web of Science: 7
Resumo

Copaifera langsdorffii Desf. (Fabaceae, Caesalpinioideae), popularly known as ``copaiba{''} or ``pau d'61eo{''}, is a species of tree that is found throughout Brazil. The leaves of this tree are used in folk medicine to treat kidney stones. Galloylquinic acid derivatives and flavonoids are the main secondary metabolites found in C. langsdorffii leaves and are likely to be responsible for the effectiveness of this treatment. As an attempt to produce a phytotherapic, we have developed a reliable HPLC-UV method for the quality control of C. langsdorffii leaves. Phenolic compounds were extracted from C. langsdorffii leaves using 70% aqueous ethanol as the extraction solvent. HPLC-UV analyses were carried out on a Synergi Polar-RP column (100 x 3.0 mm, 2.5 pm), and the mobile phase was made up of formic acid-water (0.1:99.9, solvent A), and isopropanol-methanol-acetonitrile (5:40:60, solvent B). The elution gradient was A:B (90:10 to 85:15) in 8.0 min, followed by A:B (85:15 to 64:36) up to 30.0 min, using a flow rate of 0.7 mL/min, and UV detection at 280 nm. This method was used to quantify nine galloylquinic acid derivatives and two flavonoids, which gave a good detection response and linearity in the range of 1.88-110.0 mu g/mL. Furthermore, the detection and quantification limits ranged from 0.070 to 0.752 mu g/mL, and 0.211-2.278 mu g/mL respectively, with a maximum RSD of 4.18%. The method is reliable for the quality control of C. langsdorffii raw material, its hydroethanolic extract, and could potentially be used to quantify these compounds in other Copaifera species. (AU)

Processo FAPESP: 12/01336-0 - Desenvolvimento de método analítico por cromatografia líquida de alta eficiência para quantificação de galoilquínicos nas folhas de Copaifera langsdorffii Desf. e sua aplicação na análise sazonal.
Beneficiário:Erick Vicente da Silva Motta
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 12/03852-5 - Avaliação farmacocinética do extrato bruto e de metabólitos secundários das folhas de Copaifera langsdorffii Desf.
Beneficiário:Juliana de Carvalho da Costa
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
Beneficiário:Jairo Kenupp Bastos
Linha de fomento: Auxílio à Pesquisa - Temático