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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A validated HPLC-UV method for the analysis of galloylquinic acid derivatives and flavonoids in Copaifera langsdorffii leaves

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Author(s):
da Silva Motta, Erick Vicente [1] ; da Costa, Juliana de Carvalho [1] ; Bastos, Jairo Kenupp [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1061, p. 240-247, SEP 1 2017.
Web of Science Citations: 7
Abstract

Copaifera langsdorffii Desf. (Fabaceae, Caesalpinioideae), popularly known as ``copaiba{''} or ``pau d'61eo{''}, is a species of tree that is found throughout Brazil. The leaves of this tree are used in folk medicine to treat kidney stones. Galloylquinic acid derivatives and flavonoids are the main secondary metabolites found in C. langsdorffii leaves and are likely to be responsible for the effectiveness of this treatment. As an attempt to produce a phytotherapic, we have developed a reliable HPLC-UV method for the quality control of C. langsdorffii leaves. Phenolic compounds were extracted from C. langsdorffii leaves using 70% aqueous ethanol as the extraction solvent. HPLC-UV analyses were carried out on a Synergi Polar-RP column (100 x 3.0 mm, 2.5 pm), and the mobile phase was made up of formic acid-water (0.1:99.9, solvent A), and isopropanol-methanol-acetonitrile (5:40:60, solvent B). The elution gradient was A:B (90:10 to 85:15) in 8.0 min, followed by A:B (85:15 to 64:36) up to 30.0 min, using a flow rate of 0.7 mL/min, and UV detection at 280 nm. This method was used to quantify nine galloylquinic acid derivatives and two flavonoids, which gave a good detection response and linearity in the range of 1.88-110.0 mu g/mL. Furthermore, the detection and quantification limits ranged from 0.070 to 0.752 mu g/mL, and 0.211-2.278 mu g/mL respectively, with a maximum RSD of 4.18%. The method is reliable for the quality control of C. langsdorffii raw material, its hydroethanolic extract, and could potentially be used to quantify these compounds in other Copaifera species. (AU)

FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
Grantee:Jairo Kenupp Bastos
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/03852-5 - Pharmacokinetic evaluation of the crude extract and secondary metabolites from the leaves of Copaifera langsdorffii Desf.
Grantee:Juliana de Carvalho da Costa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/01336-0 - Development of analytical method by high performance liquid chromatography for quantification of galloylquinic compounds isolated from Copaifera langsdorffii Desf. leaves and its application in seasonal analysis.
Grantee:Erick Vicente da Silva Motta
Support type: Scholarships in Brazil - Master