Monitoring cell line identity in collections of human induced pluripotent stem cells

Autor(es)

Sarafian, Raquel ^[1] ; Morato-Marques, Mariana ^[1] ; Borsoi, Juliana ^[1] ; Pereira, Lygia Veiga ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Natl Lab Embryon Stem Cells LaNCE, Dept Genet & Evolutionary Biol, Biosci Inst, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: STEM CELL RESEARCH; v. 28, p. 66-70, APR 2018.
• Citações Web of Science: 0

Resumo

The ability to reprogram somatic cells into induced pluripotent stem cells (hiPSCs) has led to the generation of large collections of cell lines from thousands of individuals with specific phenotypes, many of which will be shared among different research groups as invaluable tools for biomedical research. As hiPSC-based research involves extensive culture of many cell lines, the issue periodic cell line identification is particularly important to ensure that cell line identity remains accurate. Here we analyzed the different commercially available genotyping methods considering ease of in-house genotyping, cost and informativeness, and applied one of them in our workflow for hiPSC generation. We show that the chosen STR method was able to establish a unique DNA profile for each of the 35 individuals/hiPSC lines at the examined sites, as well as identify two discrepancies resulting from inadvertently exchanged samples. Our results highlight the importance of hiPSC line genotyping by an in-house method that allows periodic cell line identification and demonstrate that STR is a useful approach to supplement less frequent karyotyping and epigenetic evaluations. (c) 2018 The Authors. Published by Elsevier B.V. (AU)

• Processo FAPESP: 16/16076-4 - Análise funcional de mutações no gene FBN1 em células-tronco pluripotentes humanas (hPSCs)
• Beneficiário: Juliana Borsoi Sant'Ana
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/20650-8 - Avaliação de hepatócitos derivados de hiPSC como modelo para estudo da responsividade a losartan in vitro
• Beneficiário: Raquel Delgado Sarafian
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs