Effect of hypertonic saline in the pretreatment of lung donors with hemorrhagic shock

Autor(es)

Nepomuceno, Natalia Aparecida ^[1] ; de Oliveira-Braga, Karina Andrighetti ^[1] ; Ruiz, Liliane Moreira ^[1] ; Correia, Aristides Tadeu ^[1] ; Silva Pato, Eduardo Zinoni ^[2] ; da Silva, Luiz Fernando ^[3] ; Pego-Fernandes, Paulo Manuel ^[4] ; Samano, Marcos Naoyuki ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med FMUSP, Serv Cirurgia Torac, Dept Cardiopneumol, Inst Coracao, LIM 61, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Med Sch, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Med FMUSP, Pathol Dept, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Dept Cardiopneumol, Inst Coracao, LIM 61, Serv Cirur, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SURGICAL RESEARCH; v. 225, p. 181-188, MAY 2018.
• Citações Web of Science: 1

Resumo

Background: Hemorrhagic shockeinduced lung edema and inflammation are two of the main reasons for the rejection of lungs donated for transplantation. Hypertonic saline (HS) induces intravascular volume expansion and has considerable immunomodulating effects that might minimize edema. Our hypothesis is based on the use of a hypertonic solution for treatment of donors who are in shock in an attempt to increase the supply of lungs for transplantation. Methods: A total of 80 rats were allocated to four groups: one group was given an infusion of normal saline (NS; n = 20), one group received HS; n = 20, a sham group (n = 20), and a Shock group (n = 20). Half of the lungs from each group were evaluated in an ex vivo perfusion system, and the other half was used for measurements of cytokine levels and neutrophil counts. Results: In the ex vivo perfusion assessment, the pulmonary artery pressures of the animals in the NS and HS groups did not exhibit significant differences compared with those in the sham group (P > 0.05) but were lower than those in the Shock group (P < 0.01). Furthermore, the tumor necrosis factor-alpha levels and neutrophil counts were lower in the HS group than those in the Shock group (P < 0.01) and did not exhibit significant differences compared with those in either the NS and Sham groups (P > 0.05). Conclusions: We showed that HS was equivalent to isotonic saline and contributed to the treatment of lungs subjected to hemorrhagic shock. (C) 2018 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 10/52293-3 - Avaliacao ex vivo de pulmoes submetidos ao choque hipovolemico com ou sem reposicao volemica de solucao hipertonica: modelo experimental em ratos
• Beneficiário: Marcos Naoyuki Samano
• Modalidade de apoio: Auxílio à Pesquisa - Regular