Physico-chemical, antimicrobial and antioxidant properties of gelatin-chitosan based films loaded with nanoemulsions encapsulating active compounds

Autor(es)

Perez-Cordoba, Luis J. ^{[1, 2]} ; Norton, Ian T. ^[2] ; Batchelor, Hannah K. ^[3] ; Gkatzionis, Konstantinos ^[2] ; Spyropoulos, Fotios ^[2] ; Sobral, Paulo J. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Food Engn, BR-13635900 Sao Paulo - Brazil

^[2] Univ Birmingham, Sch Chem Engn, Birmingham B15 2TT, W Midlands - England

^[3] Univ Birmingham, Sch Pharm, Birmingham B15 2TT, W Midlands - England

• Tipo de documento: Artigo Científico
• Fonte: FOOD HYDROCOLLOIDS; v. 79, p. 544-559, JUN 2018.
• Citações Web of Science: 22

Resumo

The aim of this research was to develop and characterize gelatin-chitosan (4: 1) based films that incorporate nanoemulsions loaded with a range of active compounds; N-1: canola oil; N-2: alpha-tocopherol/cinnamaldehyde; N-3: alpha-tocopherol/garlic oil; or N-4: alpha-tocopherol/cinnamaldehyde and garlic oil. Nanoemulsions were prepared in a microfluidizer with pressures ranging from 69 to 100 MPa, and 3 processing cycles. Films were produced by the casting method incorporating 5 g N-1,N-2,N-3,N-4/100 g bio-polymers and using glycerol as a plasticizer, and subsequently characterized in terms of their physicochemical, antimicrobial and antioxidant properties. No differences (p > 0.05) were observed for all films in terms of moisture content (18% w/w), and thermal properties. The films' solubility in water and light transmission at 280 nm were considerably reduced as compared to the control, N-1 (15% and 60% respectively) because of the nanoemulsion incorporation. The film loaded with N-1 showed the greatest (p < 0.05) opacity, elongation at break and stiffness reduction, and was the roughest, whilst the lowest tensile strength and ability to swell were attained by films loaded with N-3 and N-4, respectively. DSC and X-ray analyses suggested compatibility among the biopolymeric-blend, and a good distribution of nanodroplets embedded into the matrix was confirmed by AFM and SEM analyses. Films loaded with nanoencapsulated active compounds (NAC) were very effective against Pseudomonas aeruginosa, and also showed high antioxidant activity. Overall, the present study offers clear evidence that these activeloaded films have the potential to be utilized as packaging material for enhancing food shelf life. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/14324-2 - Filmes à base de gelatina com agentes ativos nanoemulsionados: desenvolvimento, caracterização e aplicação em matrizes alimentícias
• Beneficiário: Luis Jaime Pérez Córdoba
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/22285-2 - Filmes ativos à base de gelatina adicionados com compostos ativos nanoemulsionados: desenvolvimento e caracterização
• Beneficiário: Luis Jaime Pérez Córdoba
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto