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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Exercise Training Restores the Cardiac Microrna-16 Levels Preventing Microvascular Rarefaction in Obese Zucker Rats

Texto completo
Autor(es):
Fernandes, Tiago [1] ; Casaes, Lilian [1] ; Soci, Ursula [1] ; Silveira, Andre [1] ; Gomes, Joao [1] ; Barretti, Diego [1] ; Roque, Fernanda [1] ; Oliveira, Edilamar [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Lab Biochem & Mol Biol Exercise, Sch Phys Educ & Sport, Av Prof Mello Moraes 65, BR-05508900 Sao Paulo, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Obesity Facts; v. 11, n. 1, p. 15-24, 2018.
Citações Web of Science: 3
Resumo

Objective: To evaluate the effects of aerobic exercise training (AET) on cardiac miRNA-16 levels and its target gene VEGF related to microvascular rarefaction in obese Zucker rats (OZR). Methods: OZR (n = 11) and lean (L; n = 10) male rats were assigned into 4 groups: OZR, trained OZR (OZRT), L and trained L (LT). Swimming exercise training lasted 60 min, 1x/day/10 weeks, with 4% body weight workload. Cardiac angiogenesis was assessed by histological analysis (periodic acid-Schiff) by calculating the capillary/fiber ratio. The protein expressions of VEGF, VEGFR2, and CD31 were evaluated by western blot. The expression of miRNA-16 was evaluated by real-time PCR. Results: Heart rate decreased in the trained groups compared to sedentary groups. The cardiac capillary/fiber ratio was reduced in OZR compared to L, LT and OZRT groups, indicating that aerobic exercise training (AET) was capable of reversing the microvascular rarefaction in the obese animals. miRNA-16 expression was increased in OZR compared to L, LT and OZRT. In contrast, its target, VEGF protein expression was 24% lower in OZR compared to L group, which has been normalized in OZRT group. VEGFR2 protein expression was increased in trained groups compared to their controls. CD31, a endothelial cells marker, showed increased expression in OZRT compared to OZR, indicating greater vascularization in OZRT group. Conclusion: AET induced cardiac angiogenesis in obese animals. This revascularization is associated with a decrease in miRNA-16 expression permissive for increased VEGF protein expression, suggesting a mechanism for potential therapeutic application in vascular diseases. (C) 2018 The Author(s) Published by S. Karger GmbH, Freiburg (AU)

Processo FAPESP: 09/18370-3 - Expressão de microRNAs no coração de ratos normotensos submetidos a diferentes volumes de treinamento físico aeróbico e seu potencial terapêutico na hipertensão
Beneficiário:Edilamar Menezes de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50673-4 - A new therapeutic approach to arterial repair and regeneration by exercise training and microRNAs targeted to vasorin
Beneficiário:Edilamar Menezes de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/17275-8 - Papel do microRNA-205 no déficit angiogênico mediado pela sinalização de FAK na hipertensão arterial: efeito do treinamento físico e perspectiva do potencial terapêutico
Beneficiário:Tiago Fernandes
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/50048-1 - Bases celulares e funcionais do exercício físico na doença cardiovascular
Beneficiário:Carlos Eduardo Negrão
Linha de fomento: Auxílio à Pesquisa - Temático