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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Leptospira interrogans outer membrane protein LipL21 is a potent inhibitor of neutrophil myeloperoxidase

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Autor(es):
Vieira, Monica L. [1, 2] ; Teixeira, Aline F. [1] ; Pidde, Giselle [3] ; Ching, Ana T. C. [3] ; Tambourgi, Denise V. [3] ; Nascimento, Ana Lucia T. O. [1] ; Herwald, Heiko [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Inst Butantan, Ctr Biotecnol, Ave Vital Brazil, Sao Paulo, SP - Brazil
[2] Lund Univ, Div Infect Med, Dept Clin Sci, Lund - Sweden
[3] Inst Butantan, Lab Imunoquim, Ave Vital Brazil, Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: VIRULENCE; v. 9, n. 1, p. 414-425, 2018.
Citações Web of Science: 3
Resumo

Leptospirosis is a widespread zoonotic and neglected infectious disease of human and veterinary concern that is caused by pathogenic Leptospira species. After entrance in the host, pathogenic leptospires evade the host natural defense mechanisms in order to propagate and disseminate to multiple organs. Myeloperoxidase is an enzyme stored in neutrophils azurophilic granules, and is released upon neutrophil activation to produce mainly hypochlorous acid, a strong oxidant and potent antimicrobial agent. In the present investigation, we studied the modulation of myeloperoxidase activity by L. interrogans serovar Copenhageni. We show that leptospires and their culture supernatants are able to inhibit both peroxidase and chlorination activities of myeloperoxidase, without interfering with neutrophil degranulation. By leptospiral outer membrane protein extraction and fractionation, we identified the proteins LipL21 and LipL45 as myeloperoxidase inhibitors, constituting new Leptospira virulence factors. Accordingly, we propose a function for the protein LipL21, one of the most expressed leptospiral outer membrane proteins. Our results show a novel innate immune evasion mechanism by which leptospires interfere with the host response in order to cope with the host oxidative stress and efficiently achieve dissemination and colonization. (AU)

Processo FAPESP: 14/50981-0 - Busca de proteínas de superfície nas sequências do genoma da Leptospira interrogans: caracterização funcional e imunológica para o entendimento de mecanismos envolvidos na patogênese de bactéria
Beneficiário:Ana Lucia Tabet Oller Do Nascimento
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/18337-4 - Modulation of hosts' coagulation and inflammation by Leptospira
Beneficiário:Mônica Larucci Vieira
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado