Subtle Alterations in Spatial Memory Induced by Amyloid Peptides Infusion in Rats

Autor(es)

Macedo, Priscila Tavares ^{[1, 2]} ; Aquino, Antonio C. Q. ^[2] ; Meurer, Ywlliane S. R. ^{[2, 3]} ; Brandao, Luiz E. M. ^[2] ; Campelo, Clarissa L. C. ^[2] ; Lima, Ramon H. ^[2] ; Costa, Marcos R. ^[1] ; Ribeiro, Alessandra M. ^[4] ; Silva, Regina H. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Norte, Brain Inst, Natal, RN - Brazil

^[2] Univ Fed Rio Grande do Norte, Physiol Dept, Memory Studies Lab, Natal, RN - Brazil

^[3] Univ Fed Sao Paulo, Pharmacol Dept, Behav Neurosci Lab, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Biosci, Lab Neurosci & Bioprospecting Nat Prod, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN AGING NEUROSCIENCE; v. 10, JAN 30 2018.
• Citações Web of Science: 0

Resumo

The cause of Alzheimer's disease (AD) remains uncertain. The accumulation of amyloid peptides (A beta) is the main pathophysiological hallmark of the disease. Spatial deficit is an important initial sign of AD, while other types of memory impairments that appear in later stages. The Barnes maze allows the detection of subtle alterations in spatial search by the analysis of use of different strategies. Previous findings showed a general performance deficit in this task following long-term (35 days) infusion of A beta, which corresponds to the moderate or severe impairments of the disease. In the present study, we evaluated the effects of a low-dose 15-day long treatment with A beta peptides on spatial and non-spatial strategies of rats tested in the Barnes maze. A beta peptides (0.5 mu L/site/day; 30 pmoL solution of A beta 1-40:A beta 1-42 10: 1) or saline were bilaterally infused into the CA1 (on the first treatment day) and intraventricularly (on the following 15 days) in 6-month-old Wistar male rats. A beta infusion induced a deficit in the performance (increased latency and distance traveled to reach the target compared to saline group). In addition, a significant association between treatment and search strategy in the retrieval trial was found: A beta group preferred the non-spatial search strategy, while saline group preferred the spatial search. In conclusion, the protocol of A beta infusion used here induced a subtle cognitive deficit that was specific to spatial aspects. Indeed, animals under A beta treatment still showed retrieval, but using non-spatial strategies. We suggest that this approach is potentially useful to the study of the initial memory deficits in early AD. (AU)

• Processo FAPESP: 15/12308-5 - Investigação dos mecanismos relacionados às diferenças de progressão dos déficits motores entre ratos wistar e SHR (Spontaneusly hypertensive rat) submetidos a modelos farmacológicos da Doença de Parkinson
• Beneficiário: Anderson Henrique França Figueredo Leão
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado