Antimicrobial peptide-loaded liquid crystalline precursor bioadhesive system for the prevention of dental caries

Autor(es)

Aida, Kelly Limi ^[1] ; Kreling, Paula Fernanda ^[1] ; Caiaffa, Karina Sampaio ^[2] ; Fioramonti Calixto, Giovana Maria ^[3] ; Chorilli, Marlus ^[3] ; Spolidorio, Denise M. P. ^[4] ; Santos-Filho, Norival Alves ^{[5, 6]} ; Cilli, Eduardo Maffud ^[5] ; Duque, Cristiane ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, R Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Aracatuba Dent Sch, Dept Endodont, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Dept Drugs & Med, Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ, UNESP, Araraquara Dent Sch, Dept Physiol & Pathol, Aracatuba, SP - Brazil

^[5] Sao Paulo State Univ, UNESP, Inst Chem, Dept Biochem & Chem Technol, Aracatuba, SP - Brazil

^[6] Sao Paulo State Univ, UNESP, Registro Expt Campus, Registro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 13, p. 3081-3091, 2018.
• Citações Web of Science: 5

Resumo

Background: Anticaries agents must interfere with the adhesion of Streptococcus mutans and its proliferation in dental biofilm, without causing host toxicity and bacterial resistance. Natural substances, including cationic antimicrobial peptides (CAMPs) and their fragments, such as beta-defensin-3 peptide fragment (D1-23), have been widely studied. However, the chemical and physical stability of CAMPs may be compromised by external factors, such as temperature and pH, reducing the period of antimicrobial activity. Methods: To overcome the aforementioned disadvantage, this study developed and characterized a drug delivery system and evaluated the cytotoxicity and effect against S. mutans biofilm of a D1-23-loaded bioadhesive liquid crystalline system (LCS). LCS was composed of oleic acid, polyoxypropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, Carbopol (R) 974P and Carbopol (R) 971P. LCS was analyzed by polarized light microscopy (PLM), rheology (viscoelasticity and flow properties) and in vitro bioadhesion. The viability of epithelial cells was evaluated. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against S. mutans were determined for D1-23 for further evaluation of the effect against S. mutans biofilm after 4 and 24 h of exposure to treatments. Results: PLM, rheology, and in vitro bioadhesion tests showed that both viscosity and bioadhesion of LCS increased after it was diluted with artificial saliva. D1-23-loaded LCS system presented better activity against S. mutans biofilm after 24 h when compared to 4 h of treatment, showing a cumulative effect. Neither LCS nor D1-23-loaded LCS presented toxicity on human epithelial cells. Conclusion: D1-23-loaded LCS is a promising drug delivery system for the prevention of dental caries. (AU)

• Processo FAPESP: 13/01565-1 - Sistemas precursores de cristais líquidos mucoadesivos para administração bucal de peptídeo antigelatinolítico associados à terapia fotodinâmica no tratamento do câncer bucal
• Beneficiário: Giovana Maria Fioramonti Calixto Balian
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/12285-0 - Avaliação do potencial antimicrobiano e citotoxicidade de fragmentos peptídicos catiônicos isolados ou combinados na prevenção da cárie dentária
• Beneficiário: Paula Fernanda Kreling Domingues
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
• Beneficiário: Glaucius Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/19235-5 - Papel antimicrobiano e imunológico de peptídeos catiônicos na prevenção da cárie precoce da infância e uma proposta de incorporação de fragmentos desses peptídeos em sistemas de nanopartículas como agentes anticárie
• Beneficiário: Cristiane Duque
• Modalidade de apoio: Auxílio à Pesquisa - Regular