Anticancer activity of VmCT1 analogs against MCF-7 cells

Autor(es)

Pedron, Cibele Nicolaski ^[1] ; Andrade, Gislaine Patricia ^[1] ; Sato, Roseli Hiromi ^[1] ; Torossian Torres, Marcelo Der ^[1] ; Cerchiaro, Giselle ^[1] ; Ribeiro, Anderson Orzari ^[1] ; Oliveira, Jr., Vani Xavier ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CHEMICAL BIOLOGY & DRUG DESIGN; v. 91, n. 2, p. 588-596, FEB 2018.
• Citações Web of Science: 4

Resumo

Antimicrobial peptides are considered promising drug candidates due to their broad range of activity. VmCT1 (Phe-Leu-Gly-Ala-Leu-Trp-Asn-Val-Ala-Lys-Ser-Val-Phe-NH2) is an -helical antimicrobial peptide that was obtained from the Vaejovis mexicanus smithi scorpion venom. Some of its analogs showed to be as antimicrobial as the wild type, and they were designed for understanding the influence of physiochemical parameters on antimicrobial and hemolytic activity. Some cationic antimicrobial peptides exhibit anticancer activity so VmCT1 analogs were tested to verify the anticancer activity of this family of peptides. The analogs were synthesized, purified, characterized, and the conformational studies were performed. The anticancer activity was assessed against MCF-7 mammary cancer cells. The results indicated that {[}Glu](7)-VmCT1-NH2, {[}Lys](3)-VmCT1-NH2, and {[}Lys](7)-VmCT1-NH2 analogs presented moderated helical tendency (0.23-0.61) and tendency of anticancer activity at 25mol/L in 24hr of experiment; and {[}Trp](9)-VmCT1-NH2 analog that presented low helical tendency and moderated anticancer activity at 50mol/L. These results demonstrated that single substitutions on VmCT1 led to different physicochemical features and could assist on the understanding of anticancer activity of this peptide family. (AU)

• Processo FAPESP: 14/18527-8 - Ftalocianinas e Naftalocianinas: síntese de macrociclos anfifílicos para aplicação em Terapia Fotodinâmica
• Beneficiário: Anderson Orzari Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/09652-9 - Danos a biomoléculas em sistema celular neuronal com desbalanço redox e metálico
• Beneficiário: Giselle Cerchiaro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/12938-6 - Peptídeos biologicamente ativos em micro-organismos patogênicos
• Beneficiário: Vani Xavier de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/04507-5 - Aplicações biológicas de novos peptídeos antimicrobianos
• Beneficiário: Marcelo Der Torossian Torres
• Modalidade de apoio: Bolsas no Brasil - Doutorado