| Full text | |
| Author(s): |
Pedron, Cibele Nicolaski
[1]
;
Andrade, Gislaine Patricia
[1]
;
Sato, Roseli Hiromi
[1]
;
Torossian Torres, Marcelo Der
[1]
;
Cerchiaro, Giselle
[1]
;
Ribeiro, Anderson Orzari
[1]
;
Oliveira, Jr., Vani Xavier
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | CHEMICAL BIOLOGY & DRUG DESIGN; v. 91, n. 2, p. 588-596, FEB 2018. |
| Web of Science Citations: | 4 |
| Abstract | |
Antimicrobial peptides are considered promising drug candidates due to their broad range of activity. VmCT1 (Phe-Leu-Gly-Ala-Leu-Trp-Asn-Val-Ala-Lys-Ser-Val-Phe-NH2) is an -helical antimicrobial peptide that was obtained from the Vaejovis mexicanus smithi scorpion venom. Some of its analogs showed to be as antimicrobial as the wild type, and they were designed for understanding the influence of physiochemical parameters on antimicrobial and hemolytic activity. Some cationic antimicrobial peptides exhibit anticancer activity so VmCT1 analogs were tested to verify the anticancer activity of this family of peptides. The analogs were synthesized, purified, characterized, and the conformational studies were performed. The anticancer activity was assessed against MCF-7 mammary cancer cells. The results indicated that {[}Glu](7)-VmCT1-NH2, {[}Lys](3)-VmCT1-NH2, and {[}Lys](7)-VmCT1-NH2 analogs presented moderated helical tendency (0.23-0.61) and tendency of anticancer activity at 25mol/L in 24hr of experiment; and {[}Trp](9)-VmCT1-NH2 analog that presented low helical tendency and moderated anticancer activity at 50mol/L. These results demonstrated that single substitutions on VmCT1 led to different physicochemical features and could assist on the understanding of anticancer activity of this peptide family. (AU) | |
| FAPESP's process: | 14/18527-8 - Phthalocyanines and Naphthalocyanines: synthesis and characterization of amphiphilic macrocyles for photodynamic therapy |
| Grantee: | Anderson Orzari Ribeiro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/09652-9 - Biomolecule damage in neural cellular system in metalic and redox inbalance |
| Grantee: | Giselle Cerchiaro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/12938-6 - Biologically active peptides against pathogenic micro-organisms |
| Grantee: | Vani Xavier de Oliveira Junior |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/04507-5 - Biological applications of new antimicrobial peptides |
| Grantee: | Marcelo Der Torossian Torres |
| Support Opportunities: | Scholarships in Brazil - Doctorate |