Biological applications of new antimicrobial peptides

• Grant number: 14/04507-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2014
• End date: August 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Vani Xavier de Oliveira Junior
• Grantee: Marcelo Der Torossian Torres
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil
• Associated scholarship(s): 16/24413-0 - Antimicrobial and antibiofilm cationic amphipathic peptides, BE.EP.DR

Abstract

Antimicrobial peptides (AMPs) are molecules with less than 100 amino acid residues, which play an important role in the immune response system of several living beings. Due to acquired resistance by various organisms such as bacteria, fungi, viruses and parasites to the forms of treatment used nowadays, the importance of searching new compounds able to eradicate them is increasing. The AMPs began to be studied recently as an option for such treatments, and they are becoming one of the most important mean of combat to the diseases caused by the organisms. This project proposes a combinatorial synthesis of antimicrobial peptides aiming to synthesize highly activity analogs that present no significant hemolytic activity. The analogs will be synthesized by the solid phase method, purified High Performance Liquid Chromatography (HPLC) and characterized by Mass Spectrometry (LC/ESI-MS). The biological tests to verify the antimicrobial activity of these compounds will be performed in strains several micro -organisms. Conformational studies will be conducted by Circular dichroism (CD) and probably by nuclear magnetic resonance (NMR). (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDRON, CIBELE NICOLASKI; ARAUJO, IRIS; DA SILVA JUNIOR, PEDRO ISMAEL; DA SILVA, FERNANDA DIAS; TOROSSIAN TORRES, MARCELO DER; OLIVEIRA JUNIOR, VANI XAVIER. Repurposing the scorpion venom peptide VmCT1 into an active peptide against Gram-negative ESKAPE pathogens. BIOORGANIC CHEMISTRY, v. 90, n. SI, SEP 2019. (14/04507-5, 17/03046-2)

TORRES, MARCELO D. T.; SOTHISELVAM, SHANMUGAPRIYA; LU, TIMOTHY K.; DE LA FUENTE-NUNEZ, CESAR. Peptide Design Principles for Antimicrobial Applications. Journal of Molecular Biology, v. 431, n. 18, SI, p. 3547-3567, AUG 23 2019. (14/04507-5, 16/24413-0)

CARDOSO, MARLON H.; CANDIDO, ELIZABETE S.; CHAN, LAI Y.; TORRES, MARCELO DER TOROSSIAN; OSHIRO, KAREN G. N.; REZENDE, SAMILLA B.; PORTO, WILLIAM F.; LU, TIMOTHY K.; DE LA FUENTE-NUNEZ, CESAR; CRAIK, DAVID J.; et al. A Computationally Designed Peptide Derived from Escherichia coli as a Potential Drug Template for Antibacterial and Antibiofilm Therapies. ACS INFECTIOUS DISEASES, v. 4, n. 12, p. 1727-1736, DEC 2018. (14/04507-5, 16/24413-0)

PEDRON, CIBELE NICOLASKI; DE OLIVEIRA, CYNTIA SILVA; DA SILVA, ADRIANA FARIAS; ANDRADE, GISLAINE PATRICIA; DA SILVA PINHAL, MARIA APARECIDA; CERCHIARO, GISELLE; DA SILVA JUNIOR, PEDRO ISMAEL; DA SILVA, FERNANDA DIAS; TORRESD, MARCELO DER TOROSSIAN; OLIVEIRA, VANI XAVIER. The effect of lysine substitutions in the biological activities of the scorpion venom peptide VmCT1. European Journal of Pharmaceutical Sciences, v. 136, AUG 1 2019. (14/04507-5, 17/03046-2)

TORRES, MARCELO D. T.; PEDRON, CIBELE N.; HIGASHIKUNI, YASUTOMI; KRAMER, ROBIN M.; CARDOSO, MARLON H.; OSHIRO, KAREN G. N.; FRANCO, OCTAVIO L.; SILVA JUNIOR, I, PEDRO; SILVA, FERNANDA D.; OLIVEIRA JUNIOR, VANI X.; et al. Structure-function-guided exploration of the antimicrobial peptide polybia-CP identifies activity determinants and generates synthetic therapeutic candidates. COMMUNICATIONS BIOLOGY, v. 1, 2018. (14/04507-5, 16/24413-0, 14/12938-6)

SILVA, ADRIANA FARIAS; TOROSSIAN TORRES, MARCELO DER; SILVA, LEANDRO SOUZA; ALVES, FLAVIO LOPES; DE SA PINHEIRO, ANA ACACIA; MIRANDA, ANTONIO; CAPURRO, MARGARETH LARA; DE LA FUENTE-NUNEZ, CESAR; OLIVEIRA, JR., VANI XAVIER. Angiotensin II-derived constrained peptides with antiplasmodial activity and suppressed vasoconstriction. SCIENTIFIC REPORTS, v. 7, OCT 30 2017. (11/10823-9, 11/11348-2, 14/04507-5, 14/12938-6)

FREIRE, KATIELLE ALBUQUERQUE; TORRES, MARCELO DER TOROSSIAN; LIMA, DANYA BANDEIRA; MONTEIRO, MARILIA LOPES; PESSOA BEZERRA DE MENEZES, RAMON ROSEO PAULA; COSTA MARTINS, ALICE MARIA; OLIVEIRA JR, VANI XAVIER. Wasp venom peptide as a new antichagasic agent. Toxicon, v. 181, p. 71-78, JUL 15 2020. (14/04507-5, 17/03046-2)

TORRES, MARCELO D. T.; PEDRON, CIBELE N.; ARAUJO, IRIS; SILVA, JR., PEDRO I.; SILVA, FERNANDA D.; OLIVEIRA, VANI X.. Decoralin Analogs with Increased Resistance to Degradation and Lower Hemolytic Activity. CHEMISTRYSELECT, v. 2, n. 1, p. 18-23, JAN 10 2017. (14/12938-6, 14/04507-5)

PEDRON, CIBELE NICOLASKI; ARAUJO, IRIS; DA SILVA JUNIOR, PEDRO ISMAEL; DA SILVA, FERNANDA DIAS; TOROSSIAN TORRES, MARCELO DER; OLIVEIRA JUNIOR, VANI XAVIER. Repurposing the scorpion venom peptide VmCT1 into an active peptide against Gram-negative ESKAPE pathogens. BIOORGANIC CHEMISTRY, v. 90, p. 9-pg., 2019-09-01. (14/04507-5, 17/03046-2)

TORRES, MARCELO D. T.; SOTHISELVAM, SHANMUGAPRIYA; LU, TIMOTHY K.; DE LA FUENTE-NUNEZ, CESAR. Peptide Design Principles for Antimicrobial Applications. Journal of Molecular Biology, v. 431, n. 18, p. 21-pg., 2019-08-23. (14/04507-5, 16/24413-0)

CANDIDO, ELIZABETE S.; CARDOSO, MARLON H.; CHAN, LAI Y.; TORRES, MARCELO D. T.; OSHIRO, KAREN G. N.; PORTO, WILLIAM F.; RIBEIRO, SUZANA M.; HANEY, EVAN F.; HANCOCK, ROBERT E. W.; LU, TIMOTHY K.; et al. Short Cationic Peptide Derived from Archaea with Dual Antibacterial Properties and Anti-Infective Potential. ACS INFECTIOUS DISEASES, v. 5, n. 7, p. 1081-1086, JUL 2019. (14/04507-5, 16/24413-0)

TORRES, MARCELO D. T.; ANDRADE, GISLAINE P.; SATO, ROSELI H.; PEDRON, CIBELE N.; MANIERI, TANIA M.; CERCHIARO, GISELLE; RIBEIRO, ANDERSON O.; DE LA FUENTE-NUNEZ, CESAR; OLIVEIRA, JR., VANI X.. Natural and redesigned wasp venom peptides with selective antitumoral activity. Beilstein Journal of Organic Chemistry, v. 14, p. 1693-1703, JUL 6 2018. (14/18527-8, 16/09652-9, 14/12938-6, 14/04507-5)

OSHIRO, KAREN G. N.; CANDIDO, ELIZABETE S.; CHAN, LAI Y.; TORRES, MARCELO D. T.; MONGES, BRUNA E. D.; RODRIGUES, SILVIA G.; PORTO, WILLIAM F.; RIBEIRO, SUZANA M.; HENRIQUES, SONIA T.; LU, TIMOTHY K.; et al. Computer-Aided Design of Mastoparan-like Peptides Enables the Generation of Nontoxic Variants with Extended Antibacterial Properties. Journal of Medicinal Chemistry, v. 62, n. 17, p. 8140-8151, SEP 12 2019. (14/04507-5, 16/24413-0)

TORRES, MARCELO D. T.; SILVA, ADRIANA F.; PEDRON, CIBELE N.; CAPURRO, MARGARETH L.; DE LA FUENTE-NUNEZ, CESAR; OLIVEIRA JUNIOR, VANI X.. Peptide Design Enables Reengineering of an Inactive Wasp Venom Peptide into Synthetic Antiplasmodial Agents. CHEMISTRYSELECT, v. 3, n. 21, p. 5859-5863, JUN 7 2018. (14/04507-5, 14/12938-6)

PEDRON, CIBELE NICOLASKI; ANDRADE, GISLAINE PATRICIA; SATO, ROSELI HIROMI; TOROSSIAN TORRES, MARCELO DER; CERCHIARO, GISELLE; RIBEIRO, ANDERSON ORZARI; OLIVEIRA, JR., VANI XAVIER. Anticancer activity of VmCT1 analogs against MCF-7 cells. CHEMICAL BIOLOGY & DRUG DESIGN, v. 91, n. 2, p. 588-596, FEB 2018. (14/18527-8, 16/09652-9, 14/12938-6, 14/04507-5)

TORRES, MARCELO DER TOROSSIAN; PEDRON, CIBELE NICOLASKI; DA SILVA LIMA, JULIA APARECIDA; DA SILVA JUNIOR, PEDRO ISMAEL; DA SILVAA, FERNANDA DIAS; OLIVEIRA JUNIOR, VANI XAVIER. Antimicrobial activity of leucine-substituted decoralin analogs with lower hemolytic activity. JOURNAL OF PEPTIDE SCIENCE, v. 23, n. 11, p. 818-823, NOV 2017. (14/04507-5, 13/12338-6, 14/12938-6)