Studies on the action of SOD1 and metal ions in the damage to biomolecules in neur...
| Grant number: | 16/09652-9 |
| Support type: | Regular Research Grants |
| Duration: | July 01, 2016 - June 30, 2018 |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry |
| Principal Investigator: | Giselle Cerchiaro |
| Grantee: | Giselle Cerchiaro |
| Home Institution: | Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil |
Abstract
Much is known about the oxidation of biomolecules and its relationship with reactive oxygen species (ROS) and intracellular redox imbalance systems. However little is known regarding the transition metals present in trace amounts (such as copper, iron and zinc) in engagement with this type of oxidation mechanism. Our research group has actively contributed to the elucidation of the role of metals in biological environment, but this seemingly simple question in neurodegeneration and redox imbalance is still unclear from the molecular point of view. This research project seek elucidation of some of these issues, noting the processes of homeostasis of copper and zinc metal (constituents of SOD1 enzyme) as well as compared to treatment with chelating agents and proteins involved in neurodegeneration (±²-amyloid), and the ability of igniting their interactions in oxidative damage, DNA damage and cell death, as well as signaling mechanisms of these processes. Thus, it is intended to provide a molecular mechanism for the relation between zinc, copper, and cellular ROS neurodegeneration.The project's main objective is the evaluation of how the redox imbalance and metal imbalance (specially copper and zinc) can cause damage to various biomolecules in neuronal cell models (hippocampal and motor neuron) submitted to ROS and neurodegeneration. (AU)