Brunetti, Andres E.
[1, 2, 3]
Marani, Mariela M.
Soldi, Rafael A.
Mendonca, Jacqueline Nakau
Cabrera, Gabriela M.
Lopes, Norberto P.
Número total de Autores: 7
Afiliação do(s) autor(es):
 Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat Bernardino Rivadavia, Div Herpetol, Ave Angel Gallardo 490, C1405DJR, Buenos Aires, DF - Argentina
 Univ Sao Paulo, Dept Phys & Chem, Fac Pharmaceut Sci Ribeirao Preto, Ave Cafe S-No, BR-14040903 Ribeirao Preto - Brazil
 Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, Ciudad Univ, Pabellon 2, 3 Piso, C1428EHA, Buenos Aires, DF - Argentina
 Consejo Nacl Invest Cient & Tecn, IPEEC, Bvd Brown 2915, U9120ACD, Puerto Madryn - Argentina
 Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Ciudad Univ, Pabellon 2, 4 Piso, C1428EHA, Buenos Aires, DF - Argentina
 Univ Buenos Aires, CONICET, Fac Ciencias Exactas & Nat, Unidad Microanal & Metodos Fis Aplicados Quim Org, Ciudad Univ, Pabellon 2, 3 Piso, C1428EHA, Buenos Aires, DF - Argentina
Número total de Afiliações: 6
Tipo de documento:
Citações Web of Science:
Peptides from skin secretions of amphibians are considered important components of their immune system and also play a relevant role in their defense mechanism against predators. Herein, by using mass spectrometry (MS), we characterize the sequence of 13 peptides from the gland secretion of the hylid tree frog, Boana punctata. Using in situ matrix-assisted laser desorption ionization imaging MS of a transverse section of the skin tissue, we show that some peptides are stored as longer molecules that are cleaved after being secreted, whereas others do not undergo any modification. Sequence comparison with peptides from other Boana species and analysis of the three-dimensional theoretical structure indicate that this cleavage depends on both the presence of a specific sequence motif and the secondary structure. The fact that peptides undergo a rapid cleavage upon secretion suggests that stored and secreted peptides may have distinct roles for anuran survival, including defense against pathogens and predators. (AU)