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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry

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Author(s):
Brunetti, Andres E. [1, 2, 3] ; Marani, Mariela M. [4] ; Soldi, Rafael A. [2] ; Mendonca, Jacqueline Nakau [2] ; Faivovich, Julian [1, 5] ; Cabrera, Gabriela M. [3, 6] ; Lopes, Norberto P. [2]
Total Authors: 7
Affiliation:
[1] Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat Bernardino Rivadavia, Div Herpetol, Ave Angel Gallardo 490, C1405DJR, Buenos Aires, DF - Argentina
[2] Univ Sao Paulo, Dept Phys & Chem, Fac Pharmaceut Sci Ribeirao Preto, Ave Cafe S-No, BR-14040903 Ribeirao Preto - Brazil
[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, Ciudad Univ, Pabellon 2, 3 Piso, C1428EHA, Buenos Aires, DF - Argentina
[4] Consejo Nacl Invest Cient & Tecn, IPEEC, Bvd Brown 2915, U9120ACD, Puerto Madryn - Argentina
[5] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Ciudad Univ, Pabellon 2, 4 Piso, C1428EHA, Buenos Aires, DF - Argentina
[6] Univ Buenos Aires, CONICET, Fac Ciencias Exactas & Nat, Unidad Microanal & Metodos Fis Aplicados Quim Org, Ciudad Univ, Pabellon 2, 3 Piso, C1428EHA, Buenos Aires, DF - Argentina
Total Affiliations: 6
Document type: Journal article
Source: ACS OMEGA; v. 3, n. 5, p. 5426-5434, MAY 2018.
Web of Science Citations: 2
Abstract

Peptides from skin secretions of amphibians are considered important components of their immune system and also play a relevant role in their defense mechanism against predators. Herein, by using mass spectrometry (MS), we characterize the sequence of 13 peptides from the gland secretion of the hylid tree frog, Boana punctata. Using in situ matrix-assisted laser desorption ionization imaging MS of a transverse section of the skin tissue, we show that some peptides are stored as longer molecules that are cleaved after being secreted, whereas others do not undergo any modification. Sequence comparison with peptides from other Boana species and analysis of the three-dimensional theoretical structure indicate that this cleavage depends on both the presence of a specific sequence motif and the secondary structure. The fact that peptides undergo a rapid cleavage upon secretion suggests that stored and secreted peptides may have distinct roles for anuran survival, including defense against pathogens and predators. (AU)

FAPESP's process: 14/50265-3 - Distribution and metabolism of natural and synthetic xenobiotics: from the comprehension of reactional process to tissue imaging generation
Grantee:Norberto Peporine Lopes
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 12/10000-5 - A multi-disciplinary approach to the study of amphibian diversification
Grantee:Taran Grant
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 14/20915-6 - Chemical ecology in anurans: characterization of volatile compounds and peptide pheromones from the skin secretions of frogs
Grantee:Andrés Eduardo Brunetti
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/50741-7 - Diversity and conservation of Brazilian amphibians
Grantee:Célio Fernando Baptista Haddad
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 17/23725-1 - Genomic analysis of skin symbiotic bacteria in frogs as potential source of volatile compounds
Grantee:Andrés Eduardo Brunetti
Support type: Scholarships abroad - Research Internship - Post-doctor