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Genomic analysis of skin symbiotic bacteria in frogs as potential source of volatile compounds

Grant number: 17/23725-1
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): February 28, 2018
Effective date (End): August 27, 2018
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Norberto Peporine Lopes
Grantee:Andrés Eduardo Brunetti
Supervisor abroad: Miguel Vences Y Kilfitt
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : University of Technology Braunschweig, Germany  
Associated to the scholarship:14/20915-6 - Chemical ecology in anurans: characterization of volatile compounds and peptide pheromones from the skin secretions of frogs, BP.PD


Microorganisms inhabited different regions of animal bodies and are known to participate in fundamental aspects of the host biology such as defense against pathogens and nutrition. In addition, a few studies in mammals highlight that bacteria harbored in scent glands may produce odorous compounds used by the host to communicate. In amphibians, the role of skin-associated bacteria has been examined in relation to protection against a pathogenic fungal disease, whereas others potential contributions remain unknown. The skin of amphibians has also been widely appreciated for research in organic chemistry and biochemistry because it stores a large diversity of bioactive molecules. Besides, hundreds of species from different taxonomic groups secrete volatile compounds, which in most cases are recognized for their characteristic smells. A few recent studies have made the first steps towards understanding the chemical diversity of these compounds. These studies include those published by our group and by the group of Prof. Miguel Vences. In particular, the studies of Prof. Vences were the first to demonstrate the presence of a volatile pheromone in amphibians. Despite these advances, most aspects of volatile compounds, mainly the biosynthetic origin and function, are almost completely unknown. The present project aims to survey the skin symbiotic bacteria as a potential source of volatile compounds in amphibians. Specifically, it will be based on genomic analysis of one bacteria strain that produce the same volatiles than it host. The study system may provide strong evidence of the influence of associated microbiota in amphibian odorous secretions, and also may contribute to understand the potential role of symbiotic interactions between amphibians and microbes for other secondary metabolites.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AILIN O'DONOHOE, M. E.; CELESTE LUNA, MARIA; REGUEIRA, ELEONORA; BRUNETTI, ANDRES E.; BASSO, NESTOR G.; LYNCH, JOHN D.; PEREYRA, MARTIN O.; HERMIDA, GLADYS N. Diversity and evolution of the parotoid macrogland in true toads (Anura: Bufonidae). ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, v. 187, n. 2, p. 453-478, OCT 2019. Web of Science Citations: 0.
BRUNETTI, ANDRES E.; LYRA, MARIANA L.; MELO, WEILAN G. P.; ANDRADE, LAURA E.; PALACIOS-RODRIGUEZ, PABLO; PRADO, BARBARA M.; HADDAD, CELIO F. B.; PUPO, MONICA T.; LOPES, NORBERTO P. Symbiotic skin bacteria as a source for sex-specific scents in frogs. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 116, n. 6, p. 2124-2129, FEB 5 2019. Web of Science Citations: 2.
CELESTE LUNA, MARIA; ROBERTO VASQUEZ-ALMAZAN, CARLOS; FAIVOVICH, JULIAN; EDUARDO BRUNETTI, ANDRES. Gland composition in sexually dimorphic skin structures of two species of Hylid frogs: Plectrohyla guatemalensis and Ptychohyla hypomykter. Amphibia-Reptilia, v. 40, n. 4, p. 523-529, 2019. Web of Science Citations: 0.
BRUNETTI, ANDRES E.; MARANI, MARIELA M.; SOLDI, RAFAEL A.; MENDONCA, JACQUELINE NAKAU; FAIVOVICH, JULIAN; CABRERA, GABRIELA M.; LOPES, NORBERTO P. Cleavage of Peptides from Amphibian Skin Revealed by Combining Analysis of Gland Secretion and in Situ MALDI Imaging Mass Spectrometry. ACS OMEGA, v. 3, n. 5, p. 5426-5434, MAY 2018. Web of Science Citations: 2.

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